Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~130 kDa mGluR5 protein using Mouse Anti-mGluR5 Monoclonal Antibody, Clone S75-33. Lane 1: Molecular Weight (MW) Ladder. Lane 2: Rat Brain Membrane. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-mGluR5 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~130 kDa.)
Mouse mGluR1/5 Monoclonal Antibody | anti-Grm5 antibody
mGluR1/5 Glutamate Receptor Antibody: Alkaline Phosphatase
Gene Names
Grm5; mGluR5; mGlur5
Reactivity
Mouse, Human, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Synonyms
mGluR1/5; Monoclonal Antibody; mGluR1/5 Glutamate Receptor Antibody: Alkaline Phosphatase; mGluR; metabotropic glutamate receptor 5; metabotropic glutamate receptor 1; mGlu5; mGlu1; mGluR5; mGluR1; GPRC1E; GRM5; Metabotropic glutamate receptor 5 variant F; Metabotropic glutamate receptor 5 variant G; Metabotropic glutamate receptor 5 variant H; anti-Grm5 antibody
Host
Mouse
Reactivity
Mouse, Human, Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
S75-3
Form/Format
Protein G Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
1171
Applicable Applications for anti-Grm5 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
1ug/mL (WB), 0.1-1.0ug/mL (IHC/ICC)
Conjugate
Alkaline Phosphatase
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~130 kDa mGluR5 protein using Mouse Anti-mGluR5 Monoclonal Antibody, Clone S75-33. Lane 1: Molecular Weight (MW) Ladder. Lane 2: Rat Brain Membrane. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-mGluR5 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~130 kDa.)
Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~130 kDa mGluR5 protein using Mouse Anti-mGluR5 Monoclonal Antibody, Clone S75-33. Lane 1: Molecular Weight (MW) Ladder. Lane 2: Rat Brain Membrane. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-mGluR5 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~130 kDa.)
Related Product Information for anti-Grm5 antibody
Background Info: Detects ~130kDa (both mGluR1 and mGluR5)
Scientific Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). The AMPA receptor is a non-NMDA-type ionotropic transmembrane receptor for glutamate that mediates fast synaptic transmission in the CNS. AMPARs are composed of four types of subunits, designated as GluR1, GluR2, GluR3 and GluR4, which combine to form tetramers (3, 4).
Scientific Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). The AMPA receptor is a non-NMDA-type ionotropic transmembrane receptor for glutamate that mediates fast synaptic transmission in the CNS. AMPARs are composed of four types of subunits, designated as GluR1, GluR2, GluR3 and GluR4, which combine to form tetramers (3, 4).
Product Categories/Family for anti-Grm5 antibody
References
1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA 2. www.ionchannels.org 3. Mayer M.L. (2005) Current Opinion in Neurobio. 15(3): 282-288. 4. Keinane K., et al. (1990) Science. 249: 556-560.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
128,290 Da
NCBI Official Full Name
metabotropic glutamate receptor 5
NCBI Official Synonym Full Names
glutamate receptor, metabotropic 5
NCBI Official Symbol
Grm5
NCBI Official Synonym Symbols
mGluR5; mGlur5
NCBI Protein Information
metabotropic glutamate receptor 5; metabotropic glutamate receptor 5b; metabotropic glutamate receptor (mGluR5)
UniProt Protein Name
Metabotropic glutamate receptor 5
Protein Family
UniProt Gene Name
Grm5
UniProt Synonym Gene Names
Gprc1e; Mglur5; mGluR5
UniProt Entry Name
GRM5_RAT
NCBI Description
may play a role in synaptic transmission; may interact with mGluR1, another group I metabotropic glutamate receptor [RGD, Feb 2006]
Uniprot Description
G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
Research Articles on Grm5
Similar Products
Product Notes
The Grm5 grm5 (Catalog #AAA803316) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The mGluR1/5 Glutamate Receptor Antibody: Alkaline Phosphatase reacts with Mouse, Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's mGluR1/5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). 1ug/mL (WB), 0.1-1.0ug/mL (IHC/ICC). Researchers should empirically determine the suitability of the Grm5 grm5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mGluR1/5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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