Mouse Melanoma Monoclonal Antibody | anti-PMEL antibody

Melanoma, gp100 (pMel17, Melanocyte Protein PMEL, Melanocytes Lineage-specific Antigen GP100) (MaxLight 405)

Cat. Num. AAA6193653

• Gene Names: PMEL; P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
• Reactivity: Equine, Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: Melanoma; Monoclonal Antibody; gp100 (pMel17; Melanocyte Protein PMEL; Melanocytes Lineage-specific Antigen GP100) (MaxLight 405); anti-PMEL antibody

• Host: Mouse
• Reactivity: Equine, Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: NKI-beteb
• Specificity: Recognizes human Melanoma. Species Crossreactivity: equine. Does not react with rat.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
• Sequence Length: 575

• Applicable Applications for anti-PMEL antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
• Application Notes: IHC: Formalin-fixed, paraffin-embedded sections. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.; Applications are based on unconjugated antibody.
• Immunogen: Membranes from a human melanoma metastasis. Uniprot: P40967
• Conjugate: MaxLight405
• Positive Control: SK-MEL-28 cells or Melanoma
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human melanoma tissue stained with, peroxidase-conjugate and DAB chromogen.)

Product Categories/Family for anti-PMEL antibody

Antibodies; Cancer Markers

References

1. Leonhardt RM et al. Proprotein convertases process Pmel17 during secretion. J Biol Chem 286:9321-37 (2011). Flow Cyt ; Human. 2. Leonhardt RM et al. Endoplasmic reticulum export, subcellular distribution, and fibril formation by Pmel17 require an intact N-terminal domain junction. J Biol Chem 285:16166-83 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 318037593
• NCBI GeneID: 6490
• NCBI Accession #: NP_001186982
• NCBI GenBank Nucleotide #: NP_001186982.1
• UniProt Accession #: P40967
• Molecular Weight: 90100
• NCBI Official Full Name: melanocyte protein PMEL isoform 2
• NCBI Official Synonym Full Names: premelanosome protein
• NCBI Official Symbol: PMEL
• NCBI Official Synonym Symbols: P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
• NCBI Protein Information: melanocyte protein PMEL
• UniProt Protein Name: Melanocyte protein PMEL
• Protein Family: Melanoma antigen
• UniProt Gene Name: PMEL
• UniProt Synonym Gene Names: D12S53E; PMEL17; SILV; ME20M; ME20-S; ME20S
• UniProt Entry Name: PMEL_HUMAN

NCBI Description

This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]

Uniprot Description

SILV: Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity. Belongs to the PMEL/NMB family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Vesicle

Chromosomal Location of Human Ortholog: 12q13-q14

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; plasma membrane; melanosome; integral to membrane; extracellular region

Molecular Function: protein binding

Biological Process: melanin biosynthetic process; melanosome organization and biogenesis