Western Blot (WB)
(Figure 1. Western blot analysis of MECP2 using anti-MECP2 antibody (MBS1753954).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human U87 whole cell lysatesLane 2: human Hela whole cell lysatesLane 3: human K562 whole cell lysatesLane 4: human HEK293 whole cell lysatesLane 5: human PC-3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-MECP2 antigen affinity purified monoclonal antibody (Catalog # MBS1753954) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for MECP2 at approximately 75KD. The expected band size for MECP2 is at 75KD. )
Mouse anti-Human MECP2 Monoclonal Antibody | anti-MECP2 antibody
Anti-MECP2 Antibody (monoclonal, 2G3)
Gene Names
MECP2; RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
Reactivity
Human
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
MECP2; Monoclonal Antibody; Anti-MECP2 Antibody (monoclonal; 2G3); Methyl-CpG-binding protein 2; MeCp-2 protein; MeCp2; methyl-CpG binding protein 2; anti-MECP2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
2G3
Specificity
Mouse IgG monoclonal antibody for MECP2 detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Applicable Applications for anti-MECP2 antibody
Western Blot (WB)
Application Notes
WB: 0.1-0.5ug/ml|Human|
Immunogen
E Coli-derived human MECP2 recombinant protein (Position: K119-R453).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Figure 1. Western blot analysis of MECP2 using anti-MECP2 antibody (MBS1753954).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human U87 whole cell lysatesLane 2: human Hela whole cell lysatesLane 3: human K562 whole cell lysatesLane 4: human HEK293 whole cell lysatesLane 5: human PC-3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-MECP2 antigen affinity purified monoclonal antibody (Catalog # MBS1753954) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for MECP2 at approximately 75KD. The expected band size for MECP2 is at 75KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of MECP2 using anti-MECP2 antibody (MBS1753954).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human U87 whole cell lysatesLane 2: human Hela whole cell lysatesLane 3: human K562 whole cell lysatesLane 4: human HEK293 whole cell lysatesLane 5: human PC-3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-MECP2 antigen affinity purified monoclonal antibody (Catalog # MBS1753954) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for MECP2 at approximately 75KD. The expected band size for MECP2 is at 75KD. )
Related Product Information for anti-MECP2 antibody
MECP2 (methyl CpG binding protein 2) is a gene that encodes the protein MECP2. It is mapped to Xq28. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of cognitive disability in females. Alternative splicing results in multiple transcript variants encoding different isoforms.
References
1. Amir RE, Van den Veyver IB, Wan M, Tran CQ, Francke U, Zoghbi HY(October 1999). "Rett syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2". Nat. Genet. 23 (2): 185-8.
2. Chahrour M, et al. (2008). "MECP2, a key contributor to neurological disease, activates and represses transcription". Science. 320 (5880): 1224-9.
2. Chahrour M, et al. (2008). "MECP2, a key contributor to neurological disease, activates and represses transcription". Science. 320 (5880): 1224-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
486
NCBI Official Full Name
methyl-CpG-binding protein 2 isoform 2
NCBI Official Synonym Full Names
methyl CpG binding protein 2 (Rett syndrome)
NCBI Official Symbol
MECP2
NCBI Official Synonym Symbols
RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
NCBI Protein Information
methyl-CpG-binding protein 2; meCp-2 protein
UniProt Protein Name
Methyl-CpG-binding protein 2
Protein Family
UniProt Gene Name
MECP2
UniProt Synonym Gene Names
MeCp-2 protein; MeCp2
UniProt Entry Name
MECP2_HUMAN
Similar Products
Product Notes
The MECP2 mecp2 (Catalog #AAA1753954) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-MECP2 Antibody (monoclonal, 2G3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MECP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the MECP2 mecp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MECP2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
