Testing Data #1
(For a permanent experimental record or for an easier interpretation of results when testing multiple samples, the solid red area may be cut off the bottom of the strip to prevent further band development once the positive flow control bands have appeared. A gentle stream of air can be applied to the membrane portion of the strip to assist in drying the membrane and preventing any further development. Do not wash the strip to stop the reaction. )
RAT ISOTYPING KIT Assay Kit
RAT MONOCLONAL ANTIBODY ISOTYPING TEST KIT
Applications
Isotyping Assay (IS)
Synonyms
RAT ISOTYPING KIT; RAT MONOCLONAL ANTIBODY ISOTYPING TEST KIT; RAT ISOTYPING KIT assay kit
Form/Format
Kit
Applicable Applications for RAT ISOTYPING KIT assay kit
Isotyping Assay (IS)
Technical Advice
PROBLEM
No heavy and or light-chain band appeared on the strip, but the positive flow control bands appeared.
Possible causes:
1. The antibody concentration was too low - prepare a less dilute sample and re-test.
2. No antibody was in the sample - the hybridoma is either not secreting or is not a rat monoclonal. If possible sub-clone the hybridoma and re-test.
3. Freshly diluted samples were not used - prepare fresh dilutions and re-test.
PROBLEM
Multiple heavy and light-chain bands appear on the strip.
Possible causes:
1. Antibody concentration was too high - dilute sample further and re-test.
2. For ascites, there may be small amounts of contaminating antibodies produced - dilute sample further and re-test.
3. For tissue culture supernatant, a mixed culture may be present - re-clone the hybridoma and re-test.
PROBLEM
No positive flow control bands appear.
Possible causes:
1. Sample volume was too low <150ul) - carefully dilute a fresh sample and pipette 150ul into a new development tube and re-test.
2. Strip removed from development tube too early - re-test and allow strip to react for at least 10 minutes.
No heavy and or light-chain band appeared on the strip, but the positive flow control bands appeared.
Possible causes:
1. The antibody concentration was too low - prepare a less dilute sample and re-test.
2. No antibody was in the sample - the hybridoma is either not secreting or is not a rat monoclonal. If possible sub-clone the hybridoma and re-test.
3. Freshly diluted samples were not used - prepare fresh dilutions and re-test.
PROBLEM
Multiple heavy and light-chain bands appear on the strip.
Possible causes:
1. Antibody concentration was too high - dilute sample further and re-test.
2. For ascites, there may be small amounts of contaminating antibodies produced - dilute sample further and re-test.
3. For tissue culture supernatant, a mixed culture may be present - re-clone the hybridoma and re-test.
PROBLEM
No positive flow control bands appear.
Possible causes:
1. Sample volume was too low <150ul) - carefully dilute a fresh sample and pipette 150ul into a new development tube and re-test.
2. Strip removed from development tube too early - re-test and allow strip to react for at least 10 minutes.
Reagents In The Kits
1 Desiccant vial containing rat isotyping test strips, 10 tests.
10 Capped ready-to-use lyophilized microparticle development tubes.
Instructions For Use
10 Capped ready-to-use lyophilized microparticle development tubes.
Instructions For Use
Target Species
Rat
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. Do not use components from different lots.
Shelf Life: 12 months from date of despatch.
Shelf Life: 12 months from date of despatch.
Testing Data #1
(For a permanent experimental record or for an easier interpretation of results when testing multiple samples, the solid red area may be cut off the bottom of the strip to prevent further band development once the positive flow control bands have appeared. A gentle stream of air can be applied to the membrane portion of the strip to assist in drying the membrane and preventing any further development. Do not wash the strip to stop the reaction. )
Testing Data #1
(For a permanent experimental record or for an easier interpretation of results when testing multiple samples, the solid red area may be cut off the bottom of the strip to prevent further band development once the positive flow control bands have appeared. A gentle stream of air can be applied to the membrane portion of the strip to assist in drying the membrane and preventing any further development. Do not wash the strip to stop the reaction. )
Testing Data #2
(Rat isotyping test kits (MBS239187) contain simple dipstick test strips that give accurate results in less than 10 minutes. They have a high sensitivity during very early culture and all major classes and subclasses of immunoglobulin are discernable)
Testing Data #2
(Rat isotyping test kits (MBS239187) contain simple dipstick test strips that give accurate results in less than 10 minutes. They have a high sensitivity during very early culture and all major classes and subclasses of immunoglobulin are discernable)
Related Product Information for RAT ISOTYPING KIT assay kit
Description: The rat monoclonal antibody isotyping test kit shows no cross-reactivity with bovine IgG (
Test Principle: The assay principle is based on mouse polyclonal anti-rat antibodies coupled onto coloured micro particles and equally reactive to any rat monoclonal antibody regardless of its isotype. The isotyping strip has immobilized bands of monoclonal mouse anti-rat antibodies corresponding to each of the common rat antibody isotypes (IgG1, IgG2a, IgG2b, IgG2c and IgM) and to kappa light chains only. One side of the strip bears a positive flow control band, which indicates that the antibody-coated coloured micro particles have migrated through the strip. By using these two components, a rat monoclonal antibody can be screened for isotype by simply diluting the antibody sample, pipetting the diluted sample into the development tube where it forms a complex with the antibody coated micro particles, and inserting the strip. This complex flows through the strip until it is bound by the immobilized mouse anti-rat antibody specific for the rat monoclonal's isotype and its light chain. In approximately 5-10 minutes, the micro particle complex will aggregate as blue bands in the two sections corresponding to the monoclonal antibody's isotype and its light chain. Development of the strip is complete when the positive flow control band on one side of the test strip turns blue.
Test Principle: The assay principle is based on mouse polyclonal anti-rat antibodies coupled onto coloured micro particles and equally reactive to any rat monoclonal antibody regardless of its isotype. The isotyping strip has immobilized bands of monoclonal mouse anti-rat antibodies corresponding to each of the common rat antibody isotypes (IgG1, IgG2a, IgG2b, IgG2c and IgM) and to kappa light chains only. One side of the strip bears a positive flow control band, which indicates that the antibody-coated coloured micro particles have migrated through the strip. By using these two components, a rat monoclonal antibody can be screened for isotype by simply diluting the antibody sample, pipetting the diluted sample into the development tube where it forms a complex with the antibody coated micro particles, and inserting the strip. This complex flows through the strip until it is bound by the immobilized mouse anti-rat antibody specific for the rat monoclonal's isotype and its light chain. In approximately 5-10 minutes, the micro particle complex will aggregate as blue bands in the two sections corresponding to the monoclonal antibody's isotype and its light chain. Development of the strip is complete when the positive flow control band on one side of the test strip turns blue.
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Product Notes
The RAT ISOTYPING KIT (Catalog #AAA239187) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RAT ISOTYPING KIT can be used in a range of immunoassay formats including, but not limited to, Isotyping Assay (IS). Researchers should empirically determine the suitability of the RAT ISOTYPING KIT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAT ISOTYPING KIT, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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