Rat MECA-79 Monoclonal Antibody | anti-MECA-79 antibody

MECA-79

Cat. Num. AAA4381526

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
• Synonyms: MECA-79; Monoclonal Antibody; Peripheral Node Addressin (PNAd); anti-MECA-79 antibody

• Host: Rat
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgM, kappa
• Clone Number: MECA-79
• Specificity: MECA-79 recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250 kD. It has been identified that GlcNAc-6-SO4 sulfation contributes to MECA-79 binding and the core 1 beta1,3-N-acetylglucosaminyltransferase is required for the generation of the MECA-79 epitope. MECA-79 is expressed on high endothelial venules (HEV) of lymphoid tissues, chronic inflamed tissues and rheumatoid synovia. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. MECA-79 antibody reacts with Mouse, human and many other species PNAd and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

• Applicable Applications for anti-MECA-79 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: ELISA: For coating, order antibody without BSA; FC/FACS: 0.5-1ug/million cells; IF: 0.5-1ug/ml; WB: 0.5-1.0ug/ml; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: Human or mouse tonsil.
• Immunogen: Mouse lymph node stromal cells
• Cellular Localization: Cell Surface
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Bistrup, A., et al. 2004. Am. J. Pathol. 164: 1635-164