Mouse anti-Human MDR Protein Monoclonal Antibody | anti-MRP1 antibody
MDR Protein (PE) (MRP1, Multidrug resistance-associated protein 1, ABCC1, ATP-binding cassette sub-family C member 1, Leukotriene C(4) transporter)
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot, Flow Cytometry, Functional Assay
Purity
Purified by Affinity Chromatography
Synonyms
MDR Protein; Monoclonal Antibody; MDR Protein (PE) (MRP1; Multidrug resistance-associated protein 1; ABCC1; ATP-binding cassette sub-family C member 1; Leukotriene C(4) transporter); anti-MRP1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3H406
Specificity
Recognizes human MDR protein with a defined linear epitope (aa918-924) of MRP1. Does not crossreact with human MDR1, MDR3, MRP2, MRP3, MRP4, MRP5 or MRP6 gene products. Does not crossreact with mouse mrp1.
Purity/Purification
Purified by Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-MRP1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: 1:20. Cells fixed in PBS, 0.5% paraformaldehyde for 30 minutes, followed by anti-mouse IgG labeled with FITC. Note: Block and wash with solution containing 0.1% Tween.
WB: 1:100-1:500 (Anti mouse IgG labeled with HRP).
ICC: 1:20-1:50. [PBS, 0.5% paraformaldehyde-fixed or 70% methanol-fixed cytospin preparations (2)].
IHC: FFPE sections following antigen retrieval by microwave heating in citrate (5).
Applications are based on unconjugated antibody.
WB: 1:100-1:500 (Anti mouse IgG labeled with HRP).
ICC: 1:20-1:50. [PBS, 0.5% paraformaldehyde-fixed or 70% methanol-fixed cytospin preparations (2)].
IHC: FFPE sections following antigen retrieval by microwave heating in citrate (5).
Applications are based on unconjugated antibody.
Immunogen
Non-denatured membranes prepared from the human small cell lung cancer cell line, H69AR, which highly overexpresses MRP1(1-3).
Conjugate
PE
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. Protect from light.
Product Categories/Family for anti-MRP1 antibody
References
1) Cole, S.P.C. et al. (1992). Science 258: 1650-1654. 2) Hipfner, D.R. et al. (1999). Biochim. Biophys. Acta 1461: 359-376. 3) Hipfner, D.R. et al. (1994). Cancer Res. 54: 5788-5792. 4) Hipfner, D.R. et al. (1996). Cancer Res. 56: 3307-3314. 5) Wright, S. et al. (1998). Clin. Cancer Res. 4: 2279-2289.
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Product Notes
The MRP1 (Catalog #AAA6120716) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MDR Protein (PE) (MRP1, Multidrug resistance-associated protein 1, ABCC1, ATP-binding cassette sub-family C member 1, Leukotriene C(4) transporter) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MDR Protein can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS). FC/FACS: 1:20. Cells fixed in PBS, 0.5% paraformaldehyde for 30 minutes, followed by anti-mouse IgG labeled with FITC. Note: Block and wash with solution containing 0.1% Tween. WB: 1:100-1:500 (Anti mouse IgG labeled with HRP). ICC: 1:20-1:50. [PBS, 0.5% paraformaldehyde-fixed or 70% methanol-fixed cytospin preparations (2)]. IHC: FFPE sections following antigen retrieval by microwave heating in citrate (5). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MRP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MDR Protein, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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