Mouse anti-Human MCP-3 Monoclonal Antibody | anti-MCP-3 antibody

Mouse Anti-Human MCP-3

Cat. Num. AAA690904

• Gene Names: CCL7; FIC; MARC; MCP3; NC28; MCP-3; SCYA6; SCYA7
• Reactivity: Human
• Purity: Protein A chromatography
• Synonyms: MCP-3; Monoclonal Antibody; Mouse Anti-Human MCP-3; Monoclonal antibody anti-human MCP-3; anti-MCP-3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Purity/Purification: Protein A chromatography
• Form/Format: Lyophilized
• Sequence Length: 99

• Application Notes: Western Blot: To detect hMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-3 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
• Antibody Generation: Monoclonal antibodies were produced in C3H/HeJ mice using recombinant human MCP-3 as the immunizing antigen. IgG1 antibody was purified from ascites fluid on a Protein A affinity column.
• Buffer: PBS
• Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-3 (100 ng/ml), a concentration of 1.0-3.0 ug/ml of this antibody is required.

Related Product Information for anti-MCP-3 antibody

MCP2 and CCL7 are two monocyte chemotactic proteins produced by human MG63 osteosarcoma cells. Both MCP2 and CCL7 are members of the CC family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP1. CCL7 also shares 58% amino acid identity with MCP2. CCL7 cDNA encodes a 99 amino acid residue precursor protein from which the Nterminal 23 amino acid residues are cleaved to generate the 76 amino acid residue mature CCL7. Mature CCL7 contains a potential Nlinked and several possible Olinked glycosylation sites. Similarly to other CC chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

NCBI and Uniprot Product Information

• NCBI GI #: 13435402
• NCBI GeneID: 6354
• NCBI Accession #: NP_006264.2
• NCBI GenBank Nucleotide #: NM_006273.3
• UniProt Accession #: P80098; Q569J6
• Molecular Weight: 11,200 Da
• NCBI Official Full Name: C-C motif chemokine 7
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 7
• NCBI Official Symbol: CCL7
• NCBI Official Synonym Symbols: FIC; MARC; MCP3; NC28; MCP-3; SCYA6; SCYA7
• NCBI Protein Information: C-C motif chemokine 7; small-inducible cytokine A7; monocyte chemotactic protein 3; monocyte chemoattractant protein 3; small inducible cytokine A7 (monocyte chemotactic protein 3)
• UniProt Protein Name: C-C motif chemokine 7
• UniProt Gene Name: CCL7
• UniProt Synonym Gene Names: MCP3; SCYA6; SCYA7; MCP-3
• UniProt Entry Name: CCL7_HUMAN

NCBI Description

This gene encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.

Subunit structure: Monomer. Ref.8

Subcellular location: Secreted.

Post-translational modification: O-glycosylated.

Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

Sequence caution: The sequence CAA50405.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA50406.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA51055.1 differs from that shown. Reason: Erroneous initiation.