Testing Data
(Formalin fixed, paraffin embedded human breast ductal carcinoma stained with Mouse anti Human MCP-1 (MBS212823) following antigen retrieval with sodium citrate buffer (pH6.0))
Mouse MCP-1 Monoclonal Antibody | anti-MCP-1 antibody
MOUSE ANTI HUMAN MCP-1
Purified IgG - lyophilised
Immunohistology - Paraffin: Maximum Dilution: 10ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 0.2; Maximum Dilution: 0.4ug/ml
Preparation
Buffer Solution
Shelf Life: 12 months from date of reconstitution.
Testing Data
(Formalin fixed, paraffin embedded human breast ductal carcinoma stained with Mouse anti Human MCP-1 (MBS212823) following antigen retrieval with sodium citrate buffer (pH6.0))
Testing Data
(Formalin fixed, paraffin embedded human breast ductal carcinoma stained with Mouse anti Human MCP-1 (MBS212823) following antigen retrieval with sodium citrate buffer (pH6.0))
Testing Data
(Formalin fixed, paraffin embedded human breast ductal carcinoma stained with Mouse anti Human MCP-1 (MBS212823) following antigen retrieval with sodium citrate buffer (pH6.0))
Testing Data
(Formalin fixed, paraffin embedded human breast ductal carcinoma stained with Mouse anti Human MCP-1 (MBS212823) following antigen retrieval with sodium citrate buffer (pH6.0))
Testing Data
(Sandwich ELISA analysis of human MCP-1 using Mouse anti Human MCP-1 as a capture reagent and biotinylated Rabbit anti Human MCP-1 (MBS220208) as a detection reagent with recombinant human MCP-1 (MBS232265) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum (green) and plasma (orange) samples are included undiluted)
Testing Data
(Sandwich ELISA analysis of human MCP-1 using Mouse anti Human MCP-1 as a capture reagent and biotinylated Rabbit anti Human MCP-1 (MBS220208) as a detection reagent with recombinant human MCP-1 (MBS232265) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum (green) and plasma (orange) samples are included undiluted)
NCBI and Uniprot Product Information
NCBI Description
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]
Uniprot Description
CCL2: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Chemokine; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted
Chromosomal Location of Human Ortholog: 17q11.2-q12
Cellular Component: extracellular space; rough endoplasmic reticulum; perinuclear region of cytoplasm; endocytic vesicle; dendrite; extracellular region; synapse; perikaryon; nerve terminal
Molecular Function: heparin binding; chemokine activity; CCR2 chemokine receptor binding; receptor binding; protein kinase activity
Biological Process: maternal process involved in pregnancy; protein amino acid phosphorylation; response to antibiotic; regulation of cell shape; monocyte chemotaxis; cell surface receptor linked signal transduction; transforming growth factor beta receptor signaling pathway; response to vitamin B3; negative regulation of neuron apoptosis; cellular homeostasis; cell adhesion; neutrophil chemotaxis; organ regeneration; positive regulation of tumor necrosis factor production; JAK-STAT cascade; response to amino acid stimulus; G-protein signaling, coupled to cyclic nucleotide second messenger; organ morphogenesis; response to ethanol; unfolded protein response, activation of signaling protein activity; cellular response to insulin stimulus; response to bacterium; response to heat; response to mechanical stimulus; positive regulation of endothelial cell proliferation; response to activity; response to progesterone stimulus; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of collagen biosynthetic process; signal transduction; chemotaxis; positive regulation of synaptic transmission; positive regulation of cellular extravasation; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; response to wounding; response to gamma radiation; angiogenesis; inflammatory response; lymphocyte chemotaxis; aging; unfolded protein response; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; cytoskeleton organization and biogenesis; viral genome replication; macrophage chemotaxis; humoral immune response; leukocyte migration during inflammatory response; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; positive regulation of leukocyte mediated cytotoxicity; negative regulation of angiogenesis; cellular protein metabolic process; maternal process involved in parturition; response to hypoxia; positive regulation of T cell activation; vascular endothelial growth factor receptor signaling pathway; astrocyte cell migration
Disease: Neural Tube Defects; Mycobacterium Tuberculosis, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To
Research Articles on MCP-1
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Product Notes
The MCP-1 ccl2 (Catalog #AAA212837) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MCP-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB). ELISA: Minimum Dilution: 1.0; Maximum Dilution: 2.0ug/ml; Immunohistology - Paraffin: Maximum Dilution: 10ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Western Blotting: Minimum Dilution: 0.2; Maximum Dilution: 0.4ug/ml. Researchers should empirically determine the suitability of the MCP-1 ccl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCP-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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