Mouse anti-Human MCFD2 Monoclonal Antibody | anti-MCFD2 antibody

MCFD2 (MCFD2, SDNSF, Multiple coagulation factor deficiency protein 2, Neural stem cell-derived neuronal survival protein) (AP)

Cat. Num. AAA6120862

• Gene Names: MCFD2; F5F8D; SDNSF; F5F8D2; LMAN1IP
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: MCFD2; Monoclonal Antibody; MCFD2 (MCFD2; SDNSF; Multiple coagulation factor deficiency protein 2; Neural stem cell-derived neuronal survival protein) (AP); Neural stem cell-derived neuronal survival protein; anti-MCFD2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 165CT13.1.6
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-MCFD2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:1000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MCFD2 Mab is generated from mouse immunized with MCFD2 recombinant protein.
• Conjugate: AP
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C with the addition of glycerol. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive.

Western Blot (WB)

(MCFD2 Antibody western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the MCFD2 antibody detected the MCFD2 protein (arrow).)

Related Product Information for anti-MCFD2 antibody

The MCFD2-LMAN1 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins. Plays a role in the secretion of coagulation factors.

Product Categories/Family for anti-MCFD2 antibody

Antibodies; Transport Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 284005451
• NCBI GeneID: 90411
• NCBI Accession #: NP_001164977.1
• NCBI GenBank Nucleotide #: NM_001171506.2
• UniProt Accession #: Q8NI22; Q53SS3; Q68D61; Q8N3M5; A8K7W2; D6W5A9; E9PD95
• Molecular Weight: 14,431 Da
• NCBI Official Full Name: multiple coagulation factor deficiency protein 2 isoform A
• NCBI Official Synonym Full Names: multiple coagulation factor deficiency 2
• NCBI Official Symbol: MCFD2
• NCBI Official Synonym Symbols: F5F8D; SDNSF; F5F8D2; LMAN1IP
• NCBI Protein Information: multiple coagulation factor deficiency protein 2; neural stem cell-derived neuronal survival protein
• UniProt Protein Name: Multiple coagulation factor deficiency protein 2
• Protein Family: Multiple coagulation factor deficiency protein
• UniProt Gene Name: MCFD2
• UniProt Synonym Gene Names: SDNSF
• UniProt Entry Name: MCFD2_HUMAN

NCBI Description

This gene encodes a soluble luminal protein with two calmodulin-like EF-hand motifs at its C-terminus. This protein forms a complex with LAMN1 (lectin mannose binding protein 1; also known as ERGIC-53) that facilitates the transport of coagulation factors V (FV) and VIII (FVIII) from the endoplasmic reticulum to the Golgi apparatus via an endoplasmic reticulum Golgi intermediate compartment (ERGIC). Mutations in this gene cause combined deficiency of FV and FVIII (F5F8D); a rare autosomal recessive bleeding disorder characterized by mild to moderate bleeding and coordinate reduction in plasma FV and FVIII levels. This protein has also been shown to maintain stem cell potential in adult central nervous system and is a marker for testicular germ cell tumors. The 3' UTR of this gene contains a transposon-like human repeat element named 'THE 1'. A processed RNA pseudogene of this gene is on chromosome 6p22.1. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jun 2010]

Uniprot Description

MCFD2: The MCFD2-LMAN1 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins. Plays a role in the secretion of coagulation factors. Defects in MCFD2 are a cause of factor V and factor VIII combined deficiency type 2 (F5F8D2); also known as multiple coagulation factor deficiency 2 (MCFD2). F5F8D2 is a blood coagulation disorder characterized by bleeding symptoms similar to those in hemophilia or parahemophilia, that are caused by single deficiency of FV or FVIII, respectively. The most common symptoms are epistaxis, menorrhagia, and excessive bleeding during or after trauma. Plasma levels of coagulation factors V and VIII are in the range of 5 to 30% of normal. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: Golgi apparatus; endoplasmic reticulum; ER-Golgi intermediate compartment

Molecular Function: calcium ion binding

Biological Process: vesicle-mediated transport; protein transport; cellular protein metabolic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification

Disease: Factor V And Factor Viii, Combined Deficiency Of, 2