Testing Data
(Published customer image: Bovine gamma delta T cells impair B. abortus replication in autologous macrophages via IFN- gamma . A. -F. Bovine macrophages were infected with B. abortus (30 bacteria:1 macrophage) and then fresh media or media containing autologous gamma delta T cells were added to infected macrophages. A., C., E. Macrophage colonization (triplicate wells/treatment) was monitored over time. *P)
Mouse anti-Sheep IFN GAMMA Monoclonal Antibody | anti-IFNG antibody
MOUSE ANTI BOVINE INTERFERON GAMMA
Purified IgG - liquid
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
ELISA: Maximum Dilution: 5ug/ml as capture antibody
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
Preparation
Buffer Solution
Shelf Life: 18 months from date of despatch.
Testing Data
(Published customer image: Bovine gamma delta T cells impair B. abortus replication in autologous macrophages via IFN- gamma . A. -F. Bovine macrophages were infected with B. abortus (30 bacteria:1 macrophage) and then fresh media or media containing autologous gamma delta T cells were added to infected macrophages. A., C., E. Macrophage colonization (triplicate wells/treatment) was monitored over time. *P)
Testing Data
(Published customer image: Bovine gamma delta T cells impair B. abortus replication in autologous macrophages via IFN- gamma . A. -F. Bovine macrophages were infected with B. abortus (30 bacteria:1 macrophage) and then fresh media or media containing autologous gamma delta T cells were added to infected macrophages. A., C., E. Macrophage colonization (triplicate wells/treatment) was monitored over time. *P)
Testing Data
(Published customer image: IFN-gamma response (log10 scale) in mesenteric lymph nodes, Peyer's Patches, popliteal LN and PBMC against GRA7, TLA and MIC3 in function of time.From: Verhelst D, De Craeye S, Entrican G, Dorny P, Cox E. Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep. BMC Vet Res. 2014 Dec 16;10(1):293.)
Testing Data
(Published customer image: IFN-gamma response (log10 scale) in mesenteric lymph nodes, Peyer's Patches, popliteal LN and PBMC against GRA7, TLA and MIC3 in function of time.From: Verhelst D, De Craeye S, Entrican G, Dorny P, Cox E. Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep. BMC Vet Res. 2014 Dec 16;10(1):293.)
Testing Data
(Published customer image: gamma delta T cells require TNF-alpha to protect against B. abortus infection. C57BL/6 mice treated with anti-TCR gamma delta mAb or hamster IgG on day -1 and day 3 post-infection with 5x104 CFUs of B. abortus 2308. Some mice were also neutralized of their TNF-alpha on days -1 and 3. Seven days after infection, A. splenic weights and B. extent of brucellae colonization were determined. The mean +/- SEM of 10 mice/group is depicted; *P)
Testing Data
(Published customer image: gamma delta T cells require TNF-alpha to protect against B. abortus infection. C57BL/6 mice treated with anti-TCR gamma delta mAb or hamster IgG on day -1 and day 3 post-infection with 5x104 CFUs of B. abortus 2308. Some mice were also neutralized of their TNF-alpha on days -1 and 3. Seven days after infection, A. splenic weights and B. extent of brucellae colonization were determined. The mean +/- SEM of 10 mice/group is depicted; *P)
Testing Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
Testing Data
(Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)
NCBI and Uniprot Product Information
Uniprot Description
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Research Articles on IFNG
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Product Notes
The IFNG ifng (Catalog #AAA215513) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI BOVINE INTERFERON GAMMA reacts with Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's IFN GAMMA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), ELISpot (ES), Flow cytometry (FC/FACS)*. ELISA: This clone may be used in a sandwich ELISA in combination with clone CC302 and recombinant bovine IFN gamma. Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. ELISA: Maximum Dilution: 5ug/ml as capture antibody Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose. Researchers should empirically determine the suitability of the IFNG ifng for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFN GAMMA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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