Mouse anti-Human MB21D1 Monoclonal Antibody | anti-MB21D1 antibody

MB21D1 Antibody

Cat. Num. AAA9217055

• Gene Names: MB21D1; cGAS; h-cGAS; C6orf150
• Reactivity: Human
• Applications: Western Blot, ELISA
• Synonyms: MB21D1; Monoclonal Antibody; MB21D1 Antibody; Cyclic GMP-AMP synthase; cGAMP synthase; cGAS; h-cGAS; 2.7.7.86; Mab-21 domain-containing protein 1; C6orf150; anti-MB21D1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1697CT136.65.30
• Specificity: This MB21D1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-185 amino acids from human MB21D1.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Sequence Length: 522

• Applicable Applications for anti-MB21D1 antibody: Western Blot (WB), ELISA
• Application Notes: WB:~~1:4000
• Cellular Location: Cell membrane; Peripheral membrane protein. Cytoplasm, cytosol. Nucleus. Note=In resting conditions, localizes at the cell membrane as a peripheral membrane protein by binding to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) (PubMed:30827685). Localization at the cell membrane is required to limit the recognition of self-DNA (PubMed:30827685). Following detection of double-stranded DNA (dsDNA), released from the cell membrane into the cytosol in order to signal (PubMed:30827685). Upon transfection with dsDNA forms punctate structures that co-localize with DNA and Beclin-1 (BECN1) (PubMed:26048138). Phosphorylation at Tyr-215 promotes cytosolic retention; translocates into the nucleus following dephosphorylation (PubMed:30356214).
• Tissue Location: Expressed in the monocytic cell line THP1.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-MB21D1 Antibody at 1:4000 dilutionLane 1: THP-1 whole cell lysateLane 2: MDA-MB-231 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-MB21D1 antibody

Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double- stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.

Function: Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) fromATP and GTP and plays a key role in innateimmunity. Catalysis involves both the formation of a2',5' phosphodiester linkage at the GpA stepand the formation of a 3',5' phosphodiester linkage at the ApG step, producingc[G(2',5')pA(3',5')p]. Acts as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production. Preferentially recognizes and binds curved long DNAs. In contrast to other mammals, human CGAS displays species-specific mechanisms of DNA recognition and produces less cyclic GMP-AMP (cGAMP), allowing a morefine-tuned response to pathogens. Has antiviral activity by sensing the presence ofdsDNA from DNA viruses in the cytoplasm. Also acts as an innate immune sensor of infection by retroviruses, such as HIV-1, by detecting the presence of reverse-transcribed DNA in the cytosol. Detection of retroviral reverse-transcribed DNA in the cytosol may be indirect and be mediated via interaction with PQBP1, which directly binds reverse-transcribed retroviral DNA. Also detects the presence of DNA from bacteria, such as M.tuberculosis. cGAMP can be transferred from producing cells to neighboring cells through gap junctions,leading to promote TMEM173/STING activation and convey immune response to connecting cells. cGAMP can also be transferred between cells by virtue of packaging within viral particles contributing to IFN-induction in newly infected cells in a cGAS-independent but TMEM173/STING-dependent manner. In addition to antiviral activity, also involved in the response to cellular stresses, such assenescence, DNA damage or genome instability.Acts as a regulator of cellular senescence by binding to cytosolic chromatin fragments that are present in senescent cells, leading to trigger type-I interferon production via TMEM173/STING and promote cellular senescence (By similarity). Also involved in the inflammatory response to genome instability and double-stranded DNA breaks: acts by localizing to micronuclei arising from genome instability. Micronuclei, which as frequently found in cancer cells, consist of chromatin surrounded by its own nuclear membrane: following breakdown of the micronuclear envelope, a process associated with chromothripsis, CGAS binds self-DNA exposed to the cytosol,leading to cGAMP synthesis and subsequent activation of TMEM173/STING and type-I interferon production. Acts as a suppressor of DNA repair in response toDNA damage: translocates to the nucleusfollowing dephosphorylation at Tyr-215 andinhibits homologous recombination repair byinteracting with PARP1, the CGAS-PARP1interaction leading to impede the formation of the PARP1-TIMELESS complex.

Product Categories/Family for anti-MB21D1 antibody

Crown Antibodies; Primary Antibodies; Cell Biology

References

Sun L.,et al.Science 339:786-791 (2013). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Mungall A.J.,et al.Nature 425:805-811 (2003). Choudhary C.,et al.Science 325:834-840 (2009). Olsen J.V.,et al.Sci. Signal. 3:RA3-RA3 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 115511030
• NCBI GeneID: 115004
• NCBI Accession #: NP_612450.2
• NCBI GenBank Nucleotide #: NM_138441.2
• UniProt Accession #: Q8N884; Q14CV6; Q32NC9; Q5SWL0; Q5SWL1; Q96E45; L0L2J9
• Molecular Weight: 58814
• NCBI Official Full Name: cyclic GMP-AMP synthase
• NCBI Official Synonym Full Names: Mab-21 domain containing 1
• NCBI Official Symbol: MB21D1
• NCBI Official Synonym Symbols: cGAS; h-cGAS; C6orf150
• NCBI Protein Information: cyclic GMP-AMP synthase
• UniProt Protein Name: Cyclic GMP-AMP synthase
• Protein Family: Cyclic GMP-AMP synthase
• UniProt Gene Name: MB21D1
• UniProt Synonym Gene Names: C6orf150; cGAMP synthase; cGAS; h-cGAS
• UniProt Entry Name: CGAS_HUMAN

Uniprot Description

MB21D1: Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.

Protein type: Enzyme, cellular metabolism; EC 2.7.7.86

Chromosomal Location of Human Ortholog: 6q13

Cellular Component: cytosol

Molecular Function: ATP binding; DNA binding; GTP binding; metal ion binding

Biological Process: activation of innate immune response; cyclic nucleotide biosynthetic process; defense response to virus; innate immune response; positive regulation of defense response to virus by host; positive regulation of interferon type I production