Mouse anti-Human MAX Monoclonal Antibody | anti-MAX antibody

MAX (Protein Max, Class D Basic Helix-loop-helix Protein 4, bHLHd4, Myc-associated Factor X, BHLHD4) (Biotin)

Cat. Num. AAA6142845

• Gene Names: MAX; bHLHd4
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MAX; Monoclonal Antibody; MAX (Protein Max; Class D Basic Helix-loop-helix Protein 4; bHLHd4; Myc-associated Factor X; BHLHD4) (Biotin); anti-MAX antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 4E10-1A9
• Specificity: Recognizes human MAX.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MAX antibody: ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF: 10ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-151 from human MAX (AAH03525) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MSDNDDIEVESDADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (42.35kD).)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to MAX on HeLa cell. [antibody concentration 10ug/ml])

Testing Data

(Detection limit for recombinant GST tagged MAX is ~0.1ng/ml as a capture antibody.)

Testing Data

(Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK14 and MAX HeLa cells were stained with MAPK14 rabbit purified polyclonal 1:1200 and MAX mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Product Categories/Family for anti-MAX antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 13097617
• NCBI GeneID: 4149
• Molecular Weight: 9,599 Da
• NCBI Official Full Name: Homo sapiens MYC associated factor X, mRNA
• NCBI Official Synonym Full Names: MYC associated factor X
• NCBI Official Symbol: MAX
• NCBI Official Synonym Symbols: bHLHd4
• NCBI Protein Information: protein max
• Protein Family: Maximin

NCBI Description

The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Mutations of this gene have been reported to be associated with hereditary pheochromocytoma. A pseudogene of this gene is located on the long arm of chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]