Mouse MARCKS Monoclonal Antibody | anti-MARCKS antibody

MARCKS (myristoylated alanine-Rich Protein Kinase C Substrate, 80K-L, FLJ14368, FLJ90045, MACS, PKCSL, PRKCSL) (FITC)

Cat. Num. AAA6177025

• Gene Names: MARCKS; MACS; 80K-L; PKCSL; PRKCSL
• Applications: Western Blot
• Purity: Purified
• Synonyms: MARCKS; Monoclonal Antibody; MARCKS (myristoylated alanine-Rich Protein Kinase C Substrate; 80K-L; FLJ14368; FLJ90045; MACS; PKCSL; PRKCSL) (FITC); myristoylated alanine-Rich Protein Kinase C Substrate; PRKCSL; anti-MARCKS antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2H4
• Specificity: Recognizes MARCKS.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-MARCKS antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: MARCKS (NP_002347, 2aa-65aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.; ; FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MARCKS monoclonal antibody (M04), clone 2H4. Western Blot analysis of MARCKS expression in Raw 264.7 (Cat # L024V1).)

Testing Data

(Detection limit for recombinant GST tagged MARCKS is approximately 0.1ng/ml as a capture antibody.)

Related Product Information for anti-MARCKS antibody

The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq]

Product Categories/Family for anti-MARCKS antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 153070260
• NCBI GeneID: 4082
• NCBI Accession #: NP_002347
• NCBI GenBank Nucleotide #: NM_002356
• UniProt Accession #: P29966
• Molecular Weight: 31kDa
• NCBI Official Full Name: myristoylated alanine-rich C-kinase substrate
• NCBI Official Synonym Full Names: myristoylated alanine rich protein kinase C substrate
• NCBI Official Symbol: MARCKS
• NCBI Official Synonym Symbols: MACS; 80K-L; PKCSL; PRKCSL
• NCBI Protein Information: myristoylated alanine-rich C-kinase substrate
• UniProt Protein Name: Myristoylated alanine-rich C-kinase substrate
• Protein Family: MARCKS-related protein
• UniProt Gene Name: MARCKS
• UniProt Synonym Gene Names: MACS; PRKCSL; MARCKS; 80K-L protein; PKCSL
• UniProt Entry Name: MARCS_HUMAN

NCBI Description

The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

MARCKS: the most prominent cellular substrate of protein kinase C. Binds calmodulin, actin, and synapsin. A filamentous actin cross-linking protein. Critical for normal brain development. Phosphorylation by PKC displaces it from the plasma membrane, inhibiting F-actin cross-linking activity. Plays a role in growth cone adhesion: its nonphosphorylated form stabilizes integrin-mediated adhesions, and its phosphorylation-triggered release from adhesions causes localized growth cone detachment. Highly expressed in immature T and B lymphocytes: its expression is highest in CD4-8- T cells and B-cell blasts generated by antigen receptor signaling. Its expression and phosphorylation are closely linked to microglial activation processes involved in pathological conditions such as neuroinflammation and neurodegeneration.

Protein type: Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 6q22.2

Cellular Component: germinal vesicle; centrosome; focal adhesion; plasma membrane; cell cortex; actin cytoskeleton

Molecular Function: calmodulin binding; actin filament binding; protein kinase C binding

Biological Process: energy reserve metabolic process; regulation of insulin secretion