Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant MAPK8 protein (Right) or empty vector (Left) detected with MAPK8 antibody)
Mouse MAPK8 Monoclonal Antibody | anti-MAPK8 antibody
MAPK8 antibody
Applications
Western Blot
Purity
MAPK8 antibody was purified by affinity chromatography.
Synonyms
MAPK8; Monoclonal Antibody; MAPK8 antibody; Monoclonal MAPK8; Anti-MAPK8; Mitogen activated protein kinase 8; JNK1; SAPK1; PRKM8; JNK; Stress-activated protein kinase 1; JNK1 alpha protein kinase; MAPK 8; MAPK-8; anti-MAPK8 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3B4
Purity/Purification
MAPK8 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Applicable Applications for anti-MAPK8 antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Biological Significance
MAPK8 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha inducedapoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochromc-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a keyrole in T cell proliferation, apoptosis and differentiation.
Cross-Reactivity
Human
Immunogen
MAPK8 antibody was raised in mouse using a recombinant protein expressed in E Coli corresponding to amino acids 1-384 of human JNK1 as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant MAPK8 protein (Right) or empty vector (Left) detected with MAPK8 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant MAPK8 protein (Right) or empty vector (Left) detected with MAPK8 antibody)
Related Product Information for anti-MAPK8 antibody
Mouse monoclonal MAPK8 antibody
Product Categories/Family for anti-MAPK8 antibody
Similar Products
Product Notes
The MAPK8 (Catalog #AAA838041) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MAPK8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the MAPK8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAPK8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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