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Western Blot (WB) (Western Blot detection against Immunogen (36.8kD).)

Mouse anti-Human MAP4 Monoclonal Antibody | anti-MAP4 antibody

MAP4 (Microtubule-associated Protein 4, MAP-4, DKFZp779A1753, MGC8617) (PE)

Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
MAP4; Monoclonal Antibody; MAP4 (Microtubule-associated Protein 4; MAP-4; DKFZp779A1753; MGC8617) (PE); anti-MAP4 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
7C9
Specificity
Recognizes human MAP4.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Sequence Length
99
Applicable Applications for anti-MAP4 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-99 from human MAP4 (NP_112147.2) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MADLSLADALTEPSPDIEGEIKRDFIATLEAEAFDDVVGETVGKTDYIPLLDVDEKTGNSESKKKPCSETSQIEDTPSSKPTLLANGGHGVEGSDTTEA
Conjugate
PE
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (36.8kD).)

Western Blot (WB) (Western Blot detection against Immunogen (36.8kD).)

Western Blot (WB)

(Western Blot analysis of MAP4 expression in transfected 293T cell line by MAP4 monoclonal antibody. Lane 1: MAP4 transfected lysate (10.4kD). Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of MAP4 expression in transfected 293T cell line by MAP4 monoclonal antibody. Lane 1: MAP4 transfected lysate (10.4kD). Lane 2: Non-transfected lysate.)

Testing Data

(Detection limit for recombinant GST tagged MAP4 is 0.1ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged MAP4 is 0.1ng/ml as a capture antibody.)
Related Product Information for anti-MAP4 antibody
Microtubule-associated protein 4 (MAP4) is a ubiquitous non-neuronal microtubule-associated protein. This protein binds, polymerizes, and stabilizes microtubules and is thought to regulate microtubule dynamics during the cell cycle. p34cdc2 kinase phosphorylation of MAP4 appears to regulate the control of microtubule reorganization at mitosis (Ookata, 1997).
Product Categories/Family for anti-MAP4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
microtubule-associated protein 4 isoform 3
UniProt Protein Name
Microtubule-associated protein 4
UniProt Gene Name
MAP4
UniProt Entry Name
MAP4_HUMAN

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Product Notes

The MAP4 map4 (Catalog #AAA6158719) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MAP4 (Microtubule-associated Protein 4, MAP-4, DKFZp779A1753, MGC8617) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAP4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MAP4 map4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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