Mouse MAP2a,b Monoclonal Antibody | anti-MAP2 antibody

MAP2a,b (Microtubule Associated Protein 2a,b) (Biotin)

Cat. Num. AAA606423

• Gene Names: MAP2; MAP2A; MAP2B; MAP2C
• Reactivity: Bovine, Chicken, Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: MAP2a; b; Monoclonal Antibody; b (Microtubule Associated Protein 2a; b) (Biotin); Anti -MAP2a; anti-MAP2 antibody

• Host: Mouse
• Reactivity: Bovine, Chicken, Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1.B.642
• Specificity: Recognizes (Mr 300kD) MAP-2 protein from bovine brain. Recognizes the high MW forms (2a & 2b) of MAP-2 but not with the low MW form (2c). Reacts with dendrites and cell bodies of neurons. Species Crossreactivity: human, rat, mouse and chicken brains.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 0.01M PBS, pH 7.4 1% BSA. 0.09% sodium azide. Labeled with Biotin.

• Applicable Applications for anti-MAP2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry and studies of neuron structure.; Dilution: Immunohistochemistry (Frozen): 1:500; Optimal dilution to be determined by researcher.
• Immunogen: MAP-2 protein from bovine brain.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-MAP2 antibody

Microtubule Associated Protein 2 exists in two high molecular weight forms (2a & 2b) and a low molecular weight form. The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. This antibody is useful in studies of neuron structure in normal and malignant brain tissue and in Alzheimer's tissue.

Product Categories/Family for anti-MAP2 antibody

Antibodies; Abs to Microtubule-associated Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 44890629
• NCBI GeneID: 4133
• UniProt Accession #: P11137; Q17S04; Q8IUX2; Q99975; Q99976
• Molecular Weight: 199,526 Da
• NCBI Official Full Name: MAP2 protein, partial
• NCBI Official Synonym Full Names: microtubule-associated protein 2
• NCBI Official Symbol: MAP2
• NCBI Official Synonym Symbols: MAP2A; MAP2B; MAP2C
• NCBI Protein Information: microtubule-associated protein 2; MAP-2
• UniProt Protein Name: Microtubule-associated protein 2
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: MAP2
• UniProt Synonym Gene Names: MAP-2
• UniProt Entry Name: MTAP2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]

Uniprot Description

MAP2: a member of the microtubule-associated protein (MAP) family. Its exact function is unknown but MAPs appear to stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. Expressed at high levels in neural tissue and the prostate. Four alternatively spliced transcript variants have been reported.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q34-q35

Cellular Component: microtubule; microtubule associated complex; cell soma; cytoplasm; nucleolus; dendritic shaft

Molecular Function: calmodulin binding; protein binding; microtubule binding; structural molecule activity

Biological Process: axonogenesis; dendrite morphogenesis; microtubule bundle formation; neurite development; central nervous system neuron development