Mouse MAP2 Monoclonal Antibody | anti-MAP2 antibody

MAP2 Antibody

Cat. Num. AAA415017

• Applications: Immunofluorescence, Western Blot
• Purity: Affinity purified
• Synonyms: MAP2; Monoclonal Antibody; MAP2 Antibody; anti-MAP2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Specificity: Rat, mouse, human and cow.
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)

• Applicable Applications for anti-MAP2 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: 1:500 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:5000
• Immunogen: Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (2). MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels, and is expressed early in development. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast t (tau) is found predominantly in neuronal axons. The HGNC name for this protein is MAP2.
• Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

References

1. Dehmelt L, Halpain S. The MAP2/Tau family of microtubule-associated proteins. Genome Biol. 6:204 (2004)