Mouse anti-Human, Mouse MAP-2 Monoclonal Antibody | anti-MAP-2 antibody

MAP-2 Monoclonal Antibody

Cat. Num. AAA3014265

• Gene Names: MAP2; MAP-2; MAP2A; MAP2B; MAP2C
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry, Immunoprecipitation
• Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MAP-2; Monoclonal Antibody; MAP-2 Monoclonal Antibody; Microtubule-associated protein 2; MAP2; anti-MAP-2 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Specificity: This antibody detects endogenous levels of MAP2 (N-terminus) and does not cross-react with related proteins
• Purity/Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1827

• Applicable Applications for anti-MAP-2 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000; ICC: 1:100-200; IP: 1:50-1:200
• Immunogen: Recombinant full length Human MAP-2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot detection of MAP-2 mAb in Mouse Brain tissue&Hela cell lysates using MAP-2 mAb (1:1000 diluted).)

Immunocytochemistry (ICC)

(Immunocytochemistry of HeLa cells using anti- MAP2 (N-terminus) antibody diluted 1:150.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of Hela cell lysates using MAP2 antibody)

Related Product Information for anti-MAP-2 antibody

MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules.

NCBI and Uniprot Product Information

• NCBI GI #: 87578396
• NCBI GeneID: 4133
• NCBI Accession #: NP_002365.3
• NCBI GenBank Nucleotide #: NP_002365.3
• UniProt Accession #: P11137
• Molecular Weight: Predicted Band Size: 202KDa; Observed Band Size: 300KDa
• NCBI Official Full Name: microtubule-associated protein 2 isoform 1
• NCBI Official Synonym Full Names: microtubule associated protein 2
• NCBI Official Symbol: MAP2
• NCBI Official Synonym Symbols: MAP-2; MAP2A; MAP2B; MAP2C
• NCBI Protein Information: microtubule-associated protein 2
• UniProt Protein Name: Microtubule-associated protein 2
• UniProt Gene Name: MAP2
• UniProt Synonym Gene Names: MAP-2
• UniProt Entry Name: MTAP2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]

Uniprot Description

MAP2: a member of the microtubule-associated protein (MAP) family. Its exact function is unknown but MAPs appear to stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. Expressed at high levels in neural tissue and the prostate. Four alternatively spliced transcript variants have been reported.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q34-q35

Cellular Component: microtubule; microtubule associated complex; cell soma; cytoplasm; nucleolus; dendritic shaft

Molecular Function: calmodulin binding; protein binding; microtubule binding; structural molecule activity

Biological Process: axonogenesis; dendrite morphogenesis; microtubule bundle formation; neurite development; central nervous system neuron development