Mouse anti-Human Mannose Associated Serine Protease 2 (MASP2) Monoclonal Antibody | anti-MASP2 antibody

Cy3-Linked Monoclonal Antibody to Mannose Associated Serine Protease 2 (MASP2)

Cat. Num. AAA2108037

• Gene Names: MASP2; sMAP; MAP19; MASP-2; MASP1P1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Mannose Associated Serine Protease 2 (MASP2); Monoclonal Antibody; Cy3-Linked Monoclonal Antibody to Mannose Associated Serine Protease 2 (MASP2); Mannose-binding protein-associated serine protease 2; mannan-Binding Lectin Serine Peptidase 2; MBL-associated serine protease 2; anti-MASP2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Specificity: The antibody is a mouse monoclonal antibody raised against MASP2. It has been selected for its ability to recognize MASP2 in immunohistochemical staining and western blotting.
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Liquid
• Concentration: >=100ug/ml (varies by lot)
• Sequence: Gly170~Thr287
• Sequence Length: 686

• Applicable Applications for anti-MASP2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Conjugation: Cy3
• Cross Reactivity: Human
• Preparation and Storage: Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.; Avoid repeated freeze/thaw cycles.; ; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 21264363
• NCBI GeneID: 10747
• NCBI Accession #: NP_006601.2
• NCBI GenBank Nucleotide #: NM_006610.3
• UniProt Accession #: O00187
• NCBI Official Full Name: mannan-binding lectin serine protease 2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: mannan binding lectin serine peptidase 2
• NCBI Official Symbol: MASP2
• NCBI Official Synonym Symbols: sMAP; MAP19; MASP-2; MASP1P1
• NCBI Protein Information: mannan-binding lectin serine protease 2
• UniProt Protein Name: Mannan-binding lectin serine protease 2
• Protein Family: Mannan-binding lectin serine protease
• UniProt Gene Name: MASP2
• UniProt Synonym Gene Names: MASP-2
• UniProt Entry Name: MASP2_HUMAN

NCBI Description

This gene encodes a member of the peptidase S1 family of serine proteases. The encoded preproprotein is proteolytically processed to generate A and B chains that heterodimerize to form the mature protease. This protease cleaves complement components C2 and C4 in order to generate C3 convertase in the lectin pathway of the complement system. The encoded protease also plays a role in the coagulation cascade through cleavage of prothrombin to form thrombin. Myocardial infarction and acute stroke patients exhibit reduced serum concentrations of the encoded protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]

Uniprot Description

MASP2: Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase. Defects in MASP2 are the cause of MASP2 deficiency (MASPD). MASPD is a disorder that results in autoimmune manifestations, recurrent severe infections, and chronic inflammatory disease. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Secreted, signal peptide; EC 3.4.21.104; Secreted

Chromosomal Location of Human Ortholog: 1p36.3-p36.2

Cellular Component: extracellular region

Molecular Function: peptidase activity; protein binding; serine-type endopeptidase activity; complement component C4b binding; calcium ion binding; calcium-dependent protein binding

Biological Process: innate immune response; proteolysis; complement activation, lectin pathway; complement activation, classical pathway; complement activation

Disease: Masp2 Deficiency