Mouse anti-Human MAN1 Monoclonal Antibody | anti-MAN1 antibody

MAN1 Mouse mAb

Cat. Num. AAA435151

• Gene Names: LEMD3; MAN1
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
• Synonyms: MAN1; Monoclonal Antibody; MAN1 Mouse mAb; Inner nuclear membrane protein Man1; integral inner nuclear membrane protein; integral inner nuclear membrane protein MAN1; LEM domain-containing protein 3; AI316861; LEMD3; anti-MAN1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 910

• Applicable Applications for anti-MAN1 antibody: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-1:5000; IP: 10 tests; FC/FACS: 1:100-1:200
• Source Note: This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human MAN1
• Preparation and Storage: Store at -20 degree C. Stable for one year from the date of shipment.

Western Blot (WB)

Immunoprecipitation (IP)

(MAN1 was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25114 of 10 ug. Western blot was performed from the immunopre-cipitate using M25114 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 100 KDaObserved band size : 100 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling MAN1 with M25114 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)

Related Product Information for anti-MAN1 antibody

This locus encodes a LEM domain-containing protein. The encoded protein functions to antagonize transforming growth factor-beta signaling at the inner nuclear membrane. Two transcript variants encoding different isoforms have been found for this gene. Mutations in this gene have been associated with osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis.

Product Categories/Family for anti-MAN1 antibody

Cell Marker; Subcellular Marker; Nucleoplasma

NCBI and Uniprot Product Information

• NCBI GI #: 264681410
• NCBI GeneID: 23592
• NCBI Accession #: NP_001161086.1
• NCBI GenBank Nucleotide #: NM_001167614.1
• UniProt Accession #: Q9Y2U8; Q9NT47; Q9NYA5
• Molecular Weight: Predicted MW: 100kDa
• NCBI Official Full Name: inner nuclear membrane protein Man1 isoform 2
• NCBI Official Synonym Full Names: LEM domain containing 3
• NCBI Official Symbol: LEMD3
• NCBI Official Synonym Symbols: MAN1
• NCBI Protein Information: inner nuclear membrane protein Man1
• UniProt Protein Name: Inner nuclear membrane protein Man1
• Protein Family: Mannan endo-1,4-beta-mannosidase
• UniProt Gene Name: LEMD3
• UniProt Synonym Gene Names: MAN1

NCBI Description

This locus encodes a LEM domain-containing protein. The encoded protein functions to antagonize transforming growth factor-beta signaling at the inner nuclear membrane. Two transcript variants encoding different isoforms have been found for this gene. Mutations in this gene have been associated with osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis.[provided by RefSeq, Nov 2009]

Uniprot Description

Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. Antagonizes TGF-beta-induced cell proliferation arrest.