MAFK recombinant protein

MAFK, 1-156aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205135

• Gene Names: MAFK; P18; NFE2U
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: MAFK; 1-156aa; Human; His tag; E Coli (Denatured); Transcription factor MafK; NFE2u; P18; MAFK recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MTTNPKPNKA LKVKKEAGEN APVLSDDELV SMSVRELNQH LRGLTKEEVT RLKQRRRTLK NRGYAASCRI KRVTQKEELE RQRVELQQEV EKLARENSSM RLELDALRSK YEALQTFART VARGPVAPSK VATTSVITIV KSTELSSTSV PFSAAS
• Sequence Length: 156

• Applicable Applications for MAFK recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for MAFK recombinant protein

MAFK belongs to the bZIP family and Maf subfamily. Members of the Maf family of basic region/leucine zipper (bZIP) transcription factors affect transcription in either a positive or negative fashion, depending on their particular protein partner and the context of the target promoter. Small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Recombinant human MAFK protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for MAFK recombinant protein

Epigenomics

References

Iwata T, et al. (1998) Cytogenet Cell Genet 82 (1-2): 88-90.; Du MJ, et al. (2008) Int J Biochem Cell Biol. 40 (8):1481-93.

NCBI and Uniprot Product Information

• NCBI GI #: 4505075
• NCBI GeneID: 7975
• NCBI Accession #: NP_002351
• NCBI GenBank Nucleotide #: NM_002360.3
• UniProt Accession #: O60675; A4D214
• Molecular Weight: 19.7 kDa (176aa)
• NCBI Official Full Name: transcription factor MafK
• NCBI Official Synonym Full Names: MAF bZIP transcription factor K
• NCBI Official Symbol: MAFK
• NCBI Official Synonym Symbols: P18; NFE2U
• NCBI Protein Information: transcription factor MafK
• UniProt Protein Name: Transcription factor MafK
• Protein Family: Transcription factor
• UniProt Gene Name: MAFK

NCBI Description

The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 recognition sites are also present in the gene promoters of 2 heme biosynthetic enzymes, porphobilinogen deaminase (PBGD; MIM 609806) and ferrochelatase (FECH; MIM 612386). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG (MIM 602020), or MafK) and p45 (MIM 601490). Both subunits are members of the activator protein-1 superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160). Maf homodimers suppress transcription at NFE2 sites.[supplied by OMIM, Nov 2008]

Uniprot Description

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed:9150357). However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites (PubMed:9150357, PubMed:8932385). Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor (PubMed:9150357).