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Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).)

Mouse Macrophage & Histiocytoma Marker Monoclonal Antibody

Macrophage & Histiocytoma Marker Mouse Monoclonal Antibody

Reactivity
Human.
Does not react with Mouse, Rat and Pig.
Others not tested
Applications
Immunohistochemistry
Synonyms
Macrophage & Histiocytoma Marker; Monoclonal Antibody; Macrophage & Histiocytoma Marker Mouse Monoclonal Antibody; anti-Macrophage & Histiocytoma Marker antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human.
Does not react with Mouse, Rat and Pig.
Others not tested
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
D11
Specificity
In Western blotting, it detects an antigen of 125kDa in human liver and 135kDa in tumors of histiocytic origin. Comparative study of this MAb and a standard CD68 MAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This MAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This MAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% azide. Also available WITHOUT azide at 1.0mg/ml.
Applicable Applications for anti-Macrophage & Histiocytoma Marker antibody
Immunohistochemistry (IHC) Formalin
Application Notes
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Cellular Localization
Cell Surface & Cytoplasmic
Immunogen
Membrane preparation from human hepatocytes
Positive Control
Liver or Histiocytoma
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).)

Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).)

Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).)
References
Rudinskaya TD, Poltoranina VS, Baranov VN, Petrovichev NN, Voytenkov BO, Tretyakov LO. D11, a novel monoclonal antibody specific for human mature macrophages and peripheral blood monocytes. Immunol Lett. 1992 Jun;33(1):1-7. Frenkel MA, Tupitsyn NN, Rudinskaia TD, Poltoranina VS, Lebedeva NB, Volkova MA, Kaletin GI. [Use of a new antimacrophage monoclonal antibody D11 in diagnosis of hemoblastosis]. [Article in Russian] Gematol Transfuziol. 1995 Jul-Aug;40(4):13-6. Tupitsyn NN, et al. Reactivity of anti-macrophage monoclonal antibody D11 in human leukemia and malignant lymphoma. Int J Cancer. 1996;68(2):160-3. Petrovichev NN, et al. Antimacrophage monoclonal antibody D11 in the diagnosis of tumors of histiocytic origin. Acta Cytol. 1997;41(2):357-63.

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Product Notes

The Macrophage & Histiocytoma Marker (Catalog #AAA438935) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Macrophage & Histiocytoma Marker Mouse Monoclonal Antibody reacts with Human. Does not react with Mouse, Rat and Pig. Others not tested and may cross-react with other species as described in the data sheet. AAA Biotech's Macrophage & Histiocytoma Marker can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Formalin. Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the Macrophage & Histiocytoma Marker for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Macrophage & Histiocytoma Marker, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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