Ly49C/F/H/I Secondary Antibody
Hamster Anti-Mouse Ly-49C/F/H/I-BIOT
Reactivity
Mouse
Applications
Flow Cytometry
Synonyms
Ly49C/F/H/I; Monoclonal Secondary Antibody; Hamster Anti-Mouse Ly-49C/F/H/I-BIOT; Hamster Anti-Mouse Ly-49C/F/H/I - Biotin; Hamster Anti-Mouse Ly49C/F/H/I; Ly49C/F/H/I secondary antibody
Host
Syrian Hamster
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Syrian Hamster IgG
Clone Number
14B11
Specificity
An epitope common to the Ly49C, Ly49F, Ly49H and Ly49I allelic/splice variants
The monoclonal antibody 14B11 reacts with murine Ly49C, Ly49F, Ly49H and Ly49I, members of the lectin-like homodimeric Ly49 family of cell surface receptors.1 Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated.2 These inhibitory receptors are also expressed on a subpopulation of mouse CD8+ T cells. The 14B11 antibody stains ~ 90% of NK1.1+CD3- splenocytes, and ~ 6% of CD8+ spleen cells from C57BL/6 mice.1 The antibody also stains 30-80% of NK1.1+CD3- splenocytes in other mouse strains tested (BALB/c, C3H, 129, SJL, AKR/J).
The monoclonal antibody 14B11 reacts with murine Ly49C, Ly49F, Ly49H and Ly49I, members of the lectin-like homodimeric Ly49 family of cell surface receptors.1 Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated.2 These inhibitory receptors are also expressed on a subpopulation of mouse CD8+ T cells. The 14B11 antibody stains ~ 90% of NK1.1+CD3- splenocytes, and ~ 6% of CD8+ spleen cells from C57BL/6 mice.1 The antibody also stains 30-80% of NK1.1+CD3- splenocytes in other mouse strains tested (BALB/c, C3H, 129, SJL, AKR/J).
Form/Format
Biotin (BIOT) Conjugate
Applicable Applications for Ly49C/F/H/I secondary antibody
Flow Cytometry
Application Notes
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Characterization
To insure acceptable performance, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Coles, M.C., C.W. McMahon, H. Takizawa, and D.H. Raulet. 2000. Memory Eur. J. Immunol. 30:236.
2. Ljunggren, H.G., and K. Karre. 1990. Immunol. Today 11:237.
3. McMahon, C., and D.H. Raulet. Personal communication.
2. Ljunggren, H.G., and K. Karre. 1990. Immunol. Today 11:237.
3. McMahon, C., and D.H. Raulet. Personal communication.
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Product Notes
The Ly49C/F/H/I (Catalog #AAA673544) is a Secondary Antibody produced from Syrian Hamster and is intended for research purposes only. The product is available for immediate purchase. The Hamster Anti-Mouse Ly-49C/F/H/I-BIOT reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Ly49C/F/H/I can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry. Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells. Researchers should empirically determine the suitability of the Ly49C/F/H/I for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ly49C/F/H/I, Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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