Mouse anti-Human LRRC5 Monoclonal Antibody | anti-LRRC8D antibody

LRRC5 (LRRC8D, Leucine-rich Repeat-containing Protein 8D, Leucine-rich Repeat-containing Protein 5, UNQ213/PRO239, FLJ10470, FLJ20403)

Cat. Num. AAA643528

• Gene Names: LRRC8D; LRRC5
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: LRRC5; Monoclonal Antibody; LRRC5 (LRRC8D; Leucine-rich Repeat-containing Protein 8D; Leucine-rich Repeat-containing Protein 5; UNQ213/PRO239; FLJ10470; FLJ20403); Anti -LRRC5 (LRRC8D; anti-LRRC8D antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3H1-1C2
• Specificity: Recognizes human LRRC5.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MVSSNFWFKYPKTCSKVEHFVSILGKCFESPWTTKALSETACEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDKKDGEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTASPFPNQ

• Applicable Applications for anti-LRRC8D antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.
• Immunogen: Full length recombinant corresponding to aa1-486 from human LRRC5 (AAH09486) with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (41.84kD).)

Western Blot (WB)

(Western Blot analysis of LRRC8D expression in transfected 293T cell line by LRRC5 monoclonal antibody.|Lane 1: LRRC8D transfected lysate (16kD).|Lane 2: Non-transfected lysate.)

Testing Data

(Detection limit for recombinant GST tagged LRRC8D is ~1ng/ml as a capture antibody.)

Product Categories/Family for anti-LRRC8D antibody

Antibodies; Abs to Leucine-rich Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 197333706
• NCBI GeneID: 55144
• NCBI Accession #: NP_001127951.1
• NCBI GenBank Nucleotide #: NM_001134479.1
• UniProt Accession #: Q7L1W4; Q6UWB2; Q9NVW3; D3DT29
• Molecular Weight: 98,201 Da
• NCBI Official Full Name: leucine-rich repeat-containing protein 8D
• NCBI Official Synonym Full Names: leucine rich repeat containing 8 family, member D
• NCBI Official Symbol: LRRC8D
• NCBI Official Synonym Symbols: LRRC5
• NCBI Protein Information: leucine-rich repeat-containing protein 8D; leucine rich repeat containing 5; leucine-rich repeat-containing 5; leucine-rich repeat-containing protein 5
• UniProt Protein Name: Leucine-rich repeat-containing protein 8D
• Protein Family: Volume-regulated anion channel
• UniProt Gene Name: LRRC8D
• UniProt Synonym Gene Names: LRRC5
• UniProt Entry Name: LRC8D_HUMAN

Uniprot Description

LRRC8D: Component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and may also conduct organic osmolytes like taurine. It is unclear whether LRRC8D constitutes a pore-forming subunit or whether it is closely associated with the pore. Mediates the import of the antibiotic blasticidin-S into cell (PubMed:24782309). Belongs to the LRRC8 family. Heterooligomer; heterooligomerizes with other LRRC8 proteins (LRRC8A, LRRC8B, LRRC8C and/or LRRC8E), possibly to form a heterohexamer

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p22.2

Cellular Component: endoplasmic reticulum membrane; membrane; plasma membrane; integral to membrane

Molecular Function: protein binding

Biological Process: ion transport