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Testing Data (Detection limit for recombinant GST tagged LIPG is 3 ng/ml as a capture antibody.)

Mouse LIPG Monoclonal Antibody | anti-LIPG antibody

LIPG (Lipase, Endothelial, EDL, EL, PRO719) (Biotin)

Gene Names
LIPG; EL; EDL; PRO719
Applications
Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
LIPG; Monoclonal Antibody; LIPG (Lipase; Endothelial; EDL; EL; PRO719) (Biotin); Lipase; PRO719; anti-LIPG antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4A9
Specificity
Recognizes LIPG.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-LIPG antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
LIPG (AAH60825.1, 21aa-500aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
SPVPFGPEGRLEDKLHKPKATQTEVKPSVRFNLRTSKDPEHEGCYLSVGHSQPLEDCSFNMTAKTFFIIHGWTMSGIFENWLHKLVSALHTREKDANVVVVDWLPLAHQLYTDAVNNTRVVGHSIARMLDWLQEKDDFSLGNVHLIGYSLGAHVAGYAGNFVKGTVGRITGLDPAGPMFEGADIHKRLSPDDADFVDVLHTYTRSFGLSIGIQMPVGHIDIYPNGGDFQPGCGLNDVLGSIAYGTITEVVKCEHERAVHLFVDSLVNQDKPSFAFQCTDSNRFKKGICLSCRKNRCNSIGYNAKKMRNKRNSKMYLKTRAGMPFRVYHYQMKIHVFSYKNMGEIEPTFYVTLYGTNADSQTLPLEIVERIEQNATNTFLVYTEEDLGDLLKIQLTWEGASQSWYNLWKEFRSYLSQPRNPGRELNIRRIRVKSGETQRKLTFCTEDPENTSISPGQELWFRKCRDGWRMKNETSPTVELP
Conjugate
Biotin
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged LIPG is 3 ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged LIPG is 3 ng/ml as a capture antibody.)

Immunoprecipitation (IP)

(Immunoprecipitation of LIPG transfected lysate using anti-LIPG monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with LIPG MaxPab rabbit polyclonal antibody.)

Immunoprecipitation (IP) (Immunoprecipitation of LIPG transfected lysate using anti-LIPG monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with LIPG MaxPab rabbit polyclonal antibody.)

Western Blot (WB)

(Western Blot analysis of LIPG expression in transfected 293T cell line by LIPG monoclonal antibody (M01), clone 4A9.Lane 1: LIPG transfected lysate (Predicted MW: 56.8 KDa).Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of LIPG expression in transfected 293T cell line by LIPG monoclonal antibody (M01), clone 4A9.Lane 1: LIPG transfected lysate (Predicted MW: 56.8 KDa).Lane 2: Non-transfected lysate.)
Related Product Information for anti-LIPG antibody
The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq]
Product Categories/Family for anti-LIPG antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
56,795 Da
NCBI Official Full Name
Lipase, endothelial
NCBI Official Synonym Full Names
lipase, endothelial
NCBI Official Symbol
LIPG
NCBI Official Synonym Symbols
EL; EDL; PRO719
NCBI Protein Information
endothelial lipase; lipoprotein lipase H; endothelial cell-derived lipase
UniProt Protein Name
Endothelial lipase
Protein Family
UniProt Gene Name
LIPG
UniProt Synonym Gene Names
EDL; EL
UniProt Entry Name
LIPE_HUMAN

NCBI Description

The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq, Jul 2008]

Uniprot Description

LIPG: Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin. Belongs to the AB hydrolase superfamily. Lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - glycerolipid; Phospholipase; Secreted; EC 3.1.1.3; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: Golgi apparatus; extracellular space; cell surface; early endosome

Molecular Function: heparin binding; lipoprotein lipase activity; phospholipase A1 activity; phospholipase activity

Biological Process: cell proliferation; cholesterol homeostasis; reverse cholesterol transport; phospholipid catabolic process; regulation of lipoprotein metabolic process; lipid metabolic process; positive regulation of cholesterol transport; response to nutrient; phospholipid homeostasis

Research Articles on LIPG

Similar Products

Product Notes

The LIPG lipg (Catalog #AAA6172595) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's LIPG can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the LIPG lipg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIPG, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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