Rabbit anti-Human HHLA2 Polyclonal Antibody | anti-HHLA2 antibody

HHLA2, NT (HHLA2, HERV-H LTR-associating protein 2, Human endogenous retrovirus-H long terminal repeat-associating protein 2) (FITC)

Cat. Num. AAA6306376

• Gene Names: HHLA2; B7H7; B7-H7
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: HHLA2; Polyclonal Antibody; NT (HHLA2; HERV-H LTR-associating protein 2; Human endogenous retrovirus-H long terminal repeat-associating protein 2) (FITC); Human endogenous retrovirus-H long terminal repeat-associating protein 2; anti-HHLA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HHLA2 at ~47kD.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-HHLA2 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: IHC-P: Formalin-fixed, paraffin embedded sections. Requires HIER using sodium citrate buffer pH6.0 prior to staining.; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 58-87 of human HHLA2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB453, CEM, HL-60, MDA-MB231 cell line lysates (35ug/lane) usingMBS642929. This demonstrates that MBS642929 detected the HHLA2 protein (arrow).)

Testing Data

(All lanes: MBS642929 (1:2000) Lane 1: human pancreas lysate Lane 2: MDA-MB-453 whole cell lysate Lysates/proteins at 20ug/lane. Secondary: IgG (HRP) Goat Anti-Rabbit. Predicted band size: 47kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma using MBS642929 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS642929 for immunohistochemistry.)

Immunohistochemistry (IHC)

(Immunohistochemistry (FFPE) analysis of HHLA2 in human pancreas tissue sections usingMBS642929. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hours at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with MBS642929 (1:25) for 1 hour at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

Product Categories/Family for anti-HHLA2 antibody

Antibodies; Infectious Disease; HERV

References

Mager, D.L., et al. Genomics 59(3):255-263(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 5901964
• NCBI GeneID: 11148
• NCBI Accession #: NP_009003.1
• NCBI GenBank Nucleotide #: NM_007072.3
• UniProt Accession #: Q9UM44; Q9NWQ6; B4DKN2; D3DN60
• NCBI Official Full Name: HERV-H LTR-associating protein 2 isoform a
• NCBI Official Synonym Full Names: HERV-H LTR-associating 2
• NCBI Official Symbol: HHLA2
• NCBI Official Synonym Symbols: B7H7; B7-H7
• NCBI Protein Information: HERV-H LTR-associating protein 2
• UniProt Protein Name: HERV-H LTR-associating protein 2
• Protein Family: HERV-H LTR-associating protein
• UniProt Gene Name: HHLA2
• UniProt Entry Name: HHLA2_HUMAN

NCBI Description

This gene encodes a protein ligand found on the surface of monocytes. The encoded protein is thought to regulate cell-mediated immunity by binding to a receptor on T lymphocytes and inhibiting the proliferation of these cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

HHLA2: Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade. Up-regulated in antigen-presenting cells in response to inflammation. Induced in dendritic cells in response to IFNG, poly(I:C) or heat-killed Listeria monocytogenes (at protein level). Interacts with TMIGD2. 2 isoforms of the human protein are produced by alternative splicing

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q13.13

Cellular Component: integral to membrane

Molecular Function: protein binding

Biological Process: positive regulation of cytokine production; T cell costimulation; positive regulation of activated T cell proliferation