Mouse LIMK1 / LIMK Monoclonal Antibody | anti-LIMK1 antibody

Mouse Monoclonal [clone 1A8] (IgG2a,k) to Human LIMK1 / LIMK

Cat. Num. AAA245864

• Gene Names: LIMK1; LIMK; LIMK-1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: LIMK1 / LIMK; Monoclonal Antibody; Mouse Monoclonal [clone 1A8] (IgG2a; k) to Human LIMK1 / LIMK; Anti-LIMK1 / LIMK Antibody (clone 1A8) IHC-plus; LIMK1; LIM domain kinase 1; Lim kinase 1; LIMK-1; LIM kinase; LIMK; Human LIMK1; anti-LIMK1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1A8
• Specificity: Human LIMK1
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 647

• Applicable Applications for anti-LIMK1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (10 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell lysates and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: LIMK1 (NP_002305, 548 a.a. ~ 648 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: LIMK1 / LIMK antibody was raised against lIMK1 (NP_002305, 548 a.a. ~ 648 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-LIMK1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(LIMK1 monoclonal antibody clone 1A8. Western blot of LIMK1 expression in NIH/3T3.)

Western Blot (WB)

(LIMK1 monoclonal antibody clone 1A8. Western blot of LIMK1 expression in PC-12.)

ELISA (EIA)

(Detection limit for recombinant GST tagged LIMK1 is approximately 0.03 ng/ml as a capture antibody.)

Related Product Information for anti-LIMK1 antibody

LIMK1, a LIMK -type protein kinase, is a component of the cellular motility machinery regulating actin filament dynamics. The protein is phosphorylated by the coiled-coil-containing protein kinase ROCK, a downstream effector of the GTPase Rho. Activated LIMK1 phosphorylates cofilin and thereby inhibits its actin-depolymerizing activity. At least two protein isoforms of 305 and 647 amino acids have been reported.

Product Categories/Family for anti-LIMK1 antibody

Family: Protein Kinase; Subfamily: LIMK

NCBI and Uniprot Product Information

• NCBI GI #: 4505001
• NCBI GeneID: 3984
• NCBI Accession #: NP_002305.1
• NCBI GenBank Nucleotide #: NM_002314.3
• UniProt Accession #: P53667; O15283; Q15820; Q15821; Q75MU3; Q9Y5Q1; B7Z6I8; D3DXF4; D3DXF5
• Molecular Weight: 68,729 Da
• NCBI Official Full Name: LIM domain kinase 1 isoform 1
• NCBI Official Synonym Full Names: LIM domain kinase 1
• NCBI Official Symbol: LIMK1
• NCBI Official Synonym Symbols: LIMK; LIMK-1
• NCBI Protein Information: LIM domain kinase 1; LIM motif-containing protein kinase
• UniProt Protein Name: LIM domain kinase 1
• Protein Family: LIM domain kinase
• UniProt Gene Name: LIMK1
• UniProt Synonym Gene Names: LIMK; LIMK-1
• UniProt Entry Name: LIMK1_HUMAN

NCBI Description

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Feb 2011]

Uniprot Description

LIMK1: a TKL kinase of the LISK family which mediates Rho signaling to cytoskeleton. Contains two N-terminal LIM motifs. Phosphorylated and activated by PAK1 and ROCK, downstream effectors of Rho. May be involved in brain development. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin and induces actin cytoskeletal changes. The LIM domain interacts with the cytoplasmic domain of NRG1. Binds ROCK1. Interacts with slingshot 1. Overexpressed in prostate tumors and prostate and breast cancer cell lines; manipulation of activity correlates with invasiveness in breast and prostate cancer models. Located within the 7q11.2 amplicon associated with metastatic prostate cancer. Loss of one copy of LIMK1 is the likely cause of visuospatial cognition defects seen in small chromosomal deletions associated with dominant Williams-Beuren syndrome Three splice variant have been identified.

Protein type: EC 2.7.11.1; Protein kinase, TKL; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); TKL group; LISK family; LIMK subfamily

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: nucleoplasm; neuron projection; focal adhesion; cytoplasm; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; zinc ion binding; protein heterodimerization activity; heat shock protein binding; ATP binding; protein kinase activity

Biological Process: nervous system development; axon guidance; positive regulation of axon extension; positive regulation of stress fiber formation; ephrin receptor signaling pathway; negative regulation of ubiquitin-protein ligase activity; innate immune response; positive regulation of actin filament bundle formation; actin cytoskeleton organization and biogenesis; signal transduction; protein amino acid phosphorylation; Rho protein signal transduction

Disease: Williams-beuren Syndrome