Mouse LIMK1 Monoclonal Antibody | anti-LIMK1 antibody

Mouse monoclonal LIMK1 antibody

Cat. Num. AAA5306636

• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: LIMK1; Monoclonal Antibody; Mouse monoclonal LIMK1 antibody; LIMK1 antibody; anti-LIMK1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 6A7
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 647

• Applicable Applications for anti-LIMK1 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: FC: 1:100; IF: 1:100; WB: 1:2000
• Immunogen: LIMK1 antibody was raised in mouse using a full length recombinant protein of human LIMK1 (NP_002305) produced in HEK293 cell as the immunogen.
• Cross-Reactivity: Human
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HEK293T cells transfected with either recombinant LIMK1 protein (red) or empty vector (blue) stained using LIMK1 antibody)

Product Categories/Family for anti-LIMK1 antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 158186665
• NCBI GeneID: 65172
• NCBI Accession #: NP_113915.2
• NCBI GenBank Nucleotide #: NM_031727.2
• UniProt Accession #: P53669
• Molecular Weight: 72,594 Da
• NCBI Official Full Name: LIM domain kinase 1
• NCBI Official Synonym Full Names: LIM domain kinase 1
• NCBI Official Symbol: Limk1
• NCBI Protein Information: LIM domain kinase 1
• UniProt Protein Name: LIM domain kinase 1
• Protein Family: LIM domain kinase
• UniProt Gene Name: Limk1
• UniProt Synonym Gene Names: Limk; LIMK-1

NCBI Description

kinase that may be involved in contol of actin dynamics in the cell [RGD, Feb 2006]

Uniprot Description

LIMK1: a TKL kinase of the LISK family which mediates Rho signaling to cytoskeleton. Contains two N-terminal LIM motifs. Phosphorylated and activated by PAK1 and ROCK, downstream effectors of Rho. May be involved in brain development. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin and induces actin cytoskeletal changes. The LIM domain interacts with the cytoplasmic domain of NRG1. Binds ROCK1. Interacts with slingshot 1. Overexpressed in prostate tumors and prostate and breast cancer cell lines; manipulation of activity correlates with invasiveness in breast and prostate cancer models. Located within the 7q11.2 amplicon associated with metastatic prostate cancer. Loss of one copy of LIMK1 is the likely cause of visuospatial cognition defects seen in small chromosomal deletions associated with dominant Williams-Beuren syndrome Three splice variant have been identified.

Protein type: EC 2.7.11.1; Kinase, protein; LIMK subfamily; LISK family; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: cytoplasm; focal adhesion; Golgi apparatus; membrane; neuron projection; nucleoplasm; nucleus; perinuclear region of cytoplasm

Molecular Function: heat shock protein binding; protein heterodimerization activity

Biological Process: negative regulation of ubiquitin-protein ligase activity; positive regulation of actin filament bundle formation; positive regulation of axon extension; positive regulation of stress fiber formation; protein amino acid phosphorylation