Testing Data
(HeLa (lane 2 and 5) and HepG2 (lane 3 and 6) cell lysates were run under reducing conditions on SDS PAGE using the V3 system and transferred to a PVDF membrane. Precision Plus molecular weight marker was run in lanes 1 and 4. Rabbit anti Human GAPDH was used as the primary antibody at a dilution of 1/1000. Mouse anti Rabbit Light Chain:HRP was used as the secondary antibody at dilutions of 1/5000 (lanes 2 and 3) and 1/10000 (lanes 5 and 6). Visualization was carried out using the ChemiDoc with automatic exposure)
Mouse anti-Rabbit LIGHT CHAIN Monoclonal Antibody
MOUSE ANTI RABBIT LIGHT CHAIN: HRP
Reactivity
Rabbit
Applications
ELISA, Western Blot
Synonyms
LIGHT CHAIN; Monoclonal Antibody; MOUSE ANTI RABBIT LIGHT CHAIN: HRP; anti-LIGHT CHAIN antibody
Host
Mouse
Reactivity
Rabbit
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
SB62a
Specificity
LIGHT CHAIN
Form/Format
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Applicable Applications for anti-LIGHT CHAIN antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: 1/4000; 1/8000
Western Blot: 1/1000; 1/10000
Western Blot: 1/1000; 1/10000
Preparation
Purified IgG from tissue culture supernatant
Preservative Stabilisers
50% Glycerol
Buffer Solution
Phosphate buffered saline
Preparation and Storage
Store at -20 degree C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch
Shelf Life: 18 months from date of despatch
Testing Data
(HeLa (lane 2 and 5) and HepG2 (lane 3 and 6) cell lysates were run under reducing conditions on SDS PAGE using the V3 system and transferred to a PVDF membrane. Precision Plus molecular weight marker was run in lanes 1 and 4. Rabbit anti Human GAPDH was used as the primary antibody at a dilution of 1/1000. Mouse anti Rabbit Light Chain:HRP was used as the secondary antibody at dilutions of 1/5000 (lanes 2 and 3) and 1/10000 (lanes 5 and 6). Visualization was carried out using the ChemiDoc with automatic exposure)
Testing Data
(HeLa (lane 2 and 5) and HepG2 (lane 3 and 6) cell lysates were run under reducing conditions on SDS PAGE using the V3 system and transferred to a PVDF membrane. Precision Plus molecular weight marker was run in lanes 1 and 4. Rabbit anti Human GAPDH was used as the primary antibody at a dilution of 1/1000. Mouse anti Rabbit Light Chain:HRP was used as the secondary antibody at dilutions of 1/5000 (lanes 2 and 3) and 1/10000 (lanes 5 and 6). Visualization was carried out using the ChemiDoc with automatic exposure)
Related Product Information for anti-LIGHT CHAIN antibody
Mouse anti Rabbit light chain antibody, clone SB62a only binds to the light chain of rabbit IgG and does not bind to the heavy chain. As such it can be used as a secondary antibody in western blotting, EIA and immunoprecipitation. This antibody is not recommended for the direct detection of reduced or denatured rabbit chains on western blots.
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Product Notes
The LIGHT CHAIN (Catalog #AAA224800) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI RABBIT LIGHT CHAIN: HRP reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's LIGHT CHAIN can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: 1/4000; 1/8000 Western Blot: 1/1000; 1/10000. Researchers should empirically determine the suitability of the LIGHT CHAIN for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIGHT CHAIN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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