Mouse anti-Human CTHRC1 Monoclonal Antibody | anti-CTHRC1 antibody

Mouse Monoclonal [clone 1G12] (IgG2a,k) to Human CTHRC1

Cat. Num. AAA249099

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: CTHRC1; Monoclonal Antibody; Mouse Monoclonal [clone 1G12] (IgG2a; k) to Human CTHRC1; Anti-CTHRC1 Antibody (clone 1G12) IHC-plus; Protein NMTC1; Human CTHRC1; anti-CTHRC1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1G12
• Specificity: Human CTHRC1
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4.
• Concentration: 0.29 mg/ml (varies by lot)
• Sequence Length: 229

• Applicable Applications for anti-CTHRC1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (3 - 10 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: CTHRC1 (NP_612464, 32 a.a. ~ 142 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: CTHRC1 antibody was raised against cTHRC1 (NP_612464, 32 a.a. ~ 142 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CTHRC1 antibody IHC staining of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-CTHRC1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(Western blot of CTHRC1 expression in transfected 293T cell line by CTHRC1 monoclonal antibody (M05), clone 1G12.)

ELISA (EIA)

(Detection limit for recombinant GST tagged CTHRC1 is 0.03 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 368711292
• NCBI GeneID: 115908
• NCBI Accession #: NP_001243028.1
• NCBI GenBank Nucleotide #: NM_001256099.1
• UniProt Accession #: Q96CG8; Q6UW91; Q8IX63; G3V141
• Molecular Weight: 24,770 Da
• NCBI Official Full Name: collagen triple helix repeat-containing protein 1 isoform 2
• NCBI Official Synonym Full Names: collagen triple helix repeat containing 1
• NCBI Official Symbol: CTHRC1
• NCBI Protein Information: collagen triple helix repeat-containing protein 1
• UniProt Protein Name: Collagen triple helix repeat-containing protein 1
• Protein Family: Collagen triple helix repeat-containing protein
• UniProt Gene Name: CTHRC1
• UniProt Entry Name: CTHR1_HUMAN

NCBI Description

This locus encodes a protein that may play a role in the cellular response to arterial injury through involvement in vascular remodeling. Mutations at this locus have been associated with Barrett esophagus and esophageal adenocarcinoma. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2012]

Uniprot Description

CTHRC1: May act as a negative regulator of collagen matrix deposition. Defects in CTHRC1 may be a cause of Barrett esophagus (BE). A condition characterized by a metaplastic change in which normal esophageal squamous epithelium is replaced by a columnar and intestinal-type epithelium. Patients with Barrett esophagus have an increased risk of esophageal adenocarcinoma. The main cause of Barrett esophagus is gastroesophageal reflux. The retrograde movement of acid and bile salts from the stomach into the esophagus causes prolonged injury to the esophageal epithelium and induces chronic esophagitis, which in turn is believed to trigger the pathologic changes. Genetic variants in CTHRC1 have been found in individuals with Barrett esophagus and are thought to contribute to disease susceptibility. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular space; collagen; cytoplasm

Molecular Function: Wnt-protein binding; frizzled binding

Biological Process: positive regulation of osteoblast differentiation; inner ear receptor stereocilium organization and biogenesis; cell migration; positive regulation of protein binding; Wnt receptor signaling pathway, planar cell polarity pathway; positive regulation of osteoblast proliferation

Disease: Barrett Esophagus