Immunofluorescence (IF)
(Lgi4 is expressed in Purkinje cells of the cerebellum when detected by antibody Clone KT18. Cryo-section was from adult mouse)
Rat LGI4 Monoclonal Antibody | anti-LGI4 antibody
LGI4 antibody
Gene Names
LGI4; LGIL3
Applications
Immunofluorescence, Western Blot
Purity
LGI4 antibody was purified by Protein G chromatography.
Synonyms
LGI4; Monoclonal Antibody; LGI4 antibody; Monoclonal LGI4; Anti-LGI4; leucine-rich repeat LGI family member 4; Leucine-rich glioma-inactivated protein 4; LGI-4; LGI 4; anti-LGI4 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
KT18
Specificity
Mouse
Purity/Purification
LGI4 antibody was purified by Protein G chromatography.
Form/Format
Supplied in PBS containing 0.1% sodium azide as a preservative
Concentration
1 mg/ml (varies by lot)
Sequence Length
537
Applicable Applications for anti-LGI4 antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes
IF: 1:100
WB: 1:500
WB: 1:500
Biological Significance
LGI4 is a secreted and glycosylated leucine-rich repeat protein. LGI4 appears to be expressed in many tissues with highest expression in brain. Senechal et al have shown high expression in the rostral migratory stream and the Purkinje cell layer of the cerebellar cortex.
Biohazard Information
This product contains sodium azide which is hazardous
Immunogen
LGI4 antibody was raised in Rat using Mouse Lgi4 peptide coupled to carrier protein as the immunogen.
Preparation and Storage
Store at 4 degree C for up to 1 month. For long term storage, aliquot and store at -20 to -80 degree C. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Lgi4 is expressed in Purkinje cells of the cerebellum when detected by antibody Clone KT18. Cryo-section was from adult mouse)
Immunofluorescence (IF)
(Lgi4 is expressed in Purkinje cells of the cerebellum when detected by antibody Clone KT18. Cryo-section was from adult mouse)
Western Blot (WB)
(Western blot of whole COS cell extracts using antibody Clone KT18. 1. Cells were transfected with HA-Oct6 as negative control; 2. Cells were transfected with Lgi4-myc expression cassette. Courtesy of Dr. Dies)
Western Blot (WB)
(Western blot of whole COS cell extracts using antibody Clone KT18. 1. Cells were transfected with HA-Oct6 as negative control; 2. Cells were transfected with Lgi4-myc expression cassette. Courtesy of Dr. Dies)
Related Product Information for anti-LGI4 antibody
Rat monoclonal LGI4 antibody
Product Categories/Family for anti-LGI4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,231 Da
NCBI Official Full Name
leucine-rich repeat LGI family member 4
NCBI Official Synonym Full Names
leucine-rich repeat LGI family, member 4
NCBI Official Symbol
LGI4
NCBI Official Synonym Symbols
LGIL3
NCBI Protein Information
leucine-rich repeat LGI family member 4
UniProt Protein Name
Leucine-rich repeat LGI family member 4
Protein Family
UniProt Gene Name
LGI4
UniProt Synonym Gene Names
LGIL3
UniProt Entry Name
LGI4_HUMAN
Uniprot Description
LGI4: 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 19q13.12|19q13.11
Cellular Component: extracellular space
Biological Process: glial cell proliferation; adult locomotory behavior; neuron maturation; myelination in the peripheral nervous system
Research Articles on LGI4
Similar Products
Product Notes
The LGI4 lgi4 (Catalog #AAA533029) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's LGI4 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). IF: 1:100 WB: 1:500. Researchers should empirically determine the suitability of the LGI4 lgi4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LGI4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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