Mouse anti-Human LCK Monoclonal Antibody | anti-LCK antibody

Anti-LCK Alexa Fluor647

• Gene Names: LCK; LSK; YT16; p56lck; pp58lck
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunocytochemistry
• Purity: The purified antibody is conjugated with Alexa Fluor647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: LCK; Monoclonal Antibody; Anti-LCK Alexa Fluor647; p56Lck; LCK proto-oncogene; Src family tyrosine kinase; anti-LCK antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: LCK-01
• Specificity: The antibody LCK-01 recognizes defined epitope (aa 22-36) of Lck, a 56kDa Src-family protein tyrosine kinase (intracellular antigen).
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Phosphate buffered saline (PBS) solution with 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-LCK antibody: Flow Cytometry (FC/FACS) (QC tested), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: FC/FACS: Recommended dilution: 1-5ug/ml. Intracellular staining.
• Conjugation: Alexa Fluor647
• Negative Species: Mouse
• Immunogen: Peptide corresponding to amino acids 22-36 in the sequence of human Lck.
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human LCK (LCK-01) Alexa Fluor 647 antibody (concentration in sample 8 ug/ml).)

Flow Cytometry (FC/FACS)

(Separation of human LCK positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human LCK (LCK-01) Alexa Fluor 647 antibody (concentration in sample 8 ug/ml).)

Related Product Information for anti-LCK antibody

Lck is a Src-family tyrosine kinase, which is essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, Lck phosphorylates the ITAM motives in its zeta subunits, establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by Lck and thereby activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. Whereas the majority of Lck is localized to the plasma membrane, there is also a significant fraction associated with the Golgi apparatus, which may contribute to Raf activation under conditions of weak stimulation through the TCR. Lck is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors.

References

*Cebecauer M, Cerny J, Horejsi V.: Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells. Biochem Biophys Res Commun. 1998 Feb 24;243(3):706-10., URL: https://www.ncbi.nlm.nih.gov/pubmed/9500981dopt=A,*Romagnoli P, Strahan D, Pelosi M, Cantagrel A, van Meerwijk JP.: A potential role for protein tyrosine kinase p56(lck) in rheumatoid arthritis synovial fluid T lymphocyte hyporesponsiveness. Int Immunol. 2001 Mar;13(3):305-12., URL: https://www.ncbi.nlm.nih.gov/pubmed/11222499dopt=,*Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation. J Immunol. 2007 Feb 15;178(4):2262-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/17277131ordin,*Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62., URL: http://www.ncbi.nlm.nih.gov/pubmed/14610046itool=
*Denny MF, Kaufman HC, Chan AC, Straus DB: The lck SH3 domain is required for activation of the mitogen-activated protein kinase pathway but not the initiation of T-cell antigen receptor signaling. J Biol Chem. 1999 Feb 19;274(8):5146-52., URL: https://www.ncbi.nlm.nih.gov/pubmed/9988764ordina,*Gruber C, Henkel M, Budach W, Belka C, Jendrossek V: Involvement of tyrosine kinase p56/Lck in apoptosis induction by anticancer drugs. Biochem Pharmacol. 2004 May 15;67(10):1859-72., URL: https://www.ncbi.nlm.nih.gov/pubmed/15130763ordin,*Hur YG, Yun Y, Won J: Rosmarinic acid induces p56lck-dependent apoptosis in Jurkat and peripheral T cells via mitochondrial pathway independent from Fas/Fas ligand interaction. J Immunol. 2004 Jan 1;172(1):79-87., URL: https://www.ncbi.nlm.nih.gov/pubmed/14688312ordin,*Lovatt M, Filby A, Parravicini V, Werlen G, Palmer E, Zamoyska R: Lck regulates the threshold of activation in primary T cells, while both Lck and Fyn contribute to the magnitude of the extracellular signal-related kinase response. Mol Cell Biol. 2006 Nov;26(22):8655-65., URL: https://www.ncbi.nlm.nih.gov/pubmed/16966372ordin,*Kim MJ, Park MT, Yoon CH, Byun JY, Lee SJ: Activation of Lck is critically required for sphingosine-induced conformational activation of Bak and mitochondrial cell death. Biochem Biophys Res Commun. 2008 May 30;370(2):353-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/18371298ordin,*Li M, Ong SS, Rajwa B, Thieu VT, Geahlen RL, Harrison ML: The SH3 domain of Lck modulates T-cell receptor-dependent activation of extracellular signal-regulated kinase through activation of Raf-1. Mol Cell Biol. 2008 Jan;28(2):630-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/17998336ordin

NCBI and Uniprot Product Information

• NCBI GI #: 112789548
• NCBI GeneID: 3932
• NCBI Accession #: NP_001036236.1
• NCBI GenBank Nucleotide #: NM_001042771.1
• UniProt Accession #: P06239; P07100; Q12850; Q13152; Q5TDH8; Q5TDH9; Q7RTZ3; Q96DW4; Q9NYT8; D3DPP8
• Molecular Weight: 58,001 Da
• NCBI Official Full Name: tyrosine-protein kinase Lck
• NCBI Official Synonym Full Names: lymphocyte-specific protein tyrosine kinase
• NCBI Official Symbol: LCK
• NCBI Official Synonym Symbols: LSK; YT16; p56lck; pp58lck
• NCBI Protein Information: tyrosine-protein kinase Lck; leukocyte C-terminal Src kinase; p56(LSTRA) protein-tyrosine kinase; t cell-specific protein-tyrosine kinase; proto-oncogene tyrosine-protein kinase LCK; lymphocyte cell-specific protein-tyrosine kinase; T-lymphocyte specific
• UniProt Protein Name: Tyrosine-protein kinase Lck
• Protein Family: Lck-interacting transmembrane adapter
• UniProt Gene Name: LCK
• UniProt Synonym Gene Names: LSK
• UniProt Entry Name: LCK_HUMAN

NCBI Description

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Lck: a tyrosine kinase of the Src family that is crucial to antigen-receptor signaling in lymphocytes. plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Binds to the cytoplasmic domain of cell surface receptors, such as CD2, CD4, CD5, CD8, CD44, CD45 and CD122. Also binds to effector molecules, such as PI4K, VAV1, RASA1, FYB and to other protein kinases including CDC2, RAF1, ZAP70 and SYK. Binds to phosphatidylinositol 3'-kinase (PI3K) from T-lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of Sam68 through its SH2 domain. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Overexpression in mice leads to thymic tumors. Aberrant expression is seen in T cell leukemias and colon cancer. The leukemic translocation t(1;7)(p34;q34) has breakpoints at the T cell receptor gene and close to the Lck promoters, can cause increased Lck expression, and in one case, point mutations. A mutated Lck has also been seen in a cell line. One patient with aberrant Lck splicing suffered from SCID-like T cell deficiency. Inhibitor: BMS-279700. Three alternatively spliced isoforms of the human proteinhave been described.

Protein type: Oncoprotein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; Protein kinase, TK; TK group; Src family

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: pericentriolar material; extrinsic to internal side of plasma membrane; endocytic vesicle; plasma membrane; immunological synapse; intercellular junction; cytosol; lipid raft

Molecular Function: protein C-terminus binding; CD8 receptor binding; identical protein binding; non-membrane spanning protein tyrosine kinase activity; protein serine/threonine phosphatase activity; protein phosphatase binding; antigen binding; protein kinase binding; ATPase binding; protein binding; protein-tyrosine kinase activity; protein complex binding; phosphoinositide 3-kinase binding; SH2 domain binding; glycoprotein binding; CD4 receptor binding; ATP binding

Biological Process: viral reproduction; nerve growth factor receptor signaling pathway; T cell receptor signaling pathway; protein amino acid phosphorylation; B cell receptor signaling pathway; positive regulation of T cell receptor signaling pathway; hemopoiesis; positive regulation of gamma-delta T cell differentiation; aging; T cell differentiation; regulation of lymphocyte activation; caspase activation; response to drug; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; cellular zinc ion homeostasis; regulation of defense response to virus by virus; regulation of cell proliferation; dephosphorylation; positive regulation of tyrosine phosphorylation of Stat5 protein; response to hydrogen peroxide; response to zinc ion; response to mechanical stimulus; release of sequestered calcium ion into cytosol; T cell costimulation; innate immune response; positive regulation of T cell activation; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Immunodeficiency 22