Rat LAMP2 Monoclonal Antibody | anti-Lamp2 antibody

LAMP2 Antibody: ATTO 390

Cat. Num. AAA801917

• Gene Names: Lamp2; Mac3; LGP-B; CD107b; Lamp-2; Lamp II; Lamp-2a; Lamp-2b; Lamp-2c
• Reactivity: Mouse, Rabbit
• Applications: Immunoprecipitation, Immunocytochemistry, Immunofluorescence
• Synonyms: LAMP2; Monoclonal Antibody; LAMP2 Antibody: ATTO 390; CD107b; Igp110; Igp2; Lamp2C; LampB; MAC3; Lysosome-associated membrane glycoprotein 2; LAMP-2; Lysosome-associated membrane protein 2; CD107 antigen-like family member B; Lysosomal membrane glycoprotein type B; LGP-B; anti-Lamp2 antibody

• Host: Rat
• Reactivity: Mouse, Rabbit
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: GL2A7
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 415

• Applicable Applications for anti-Lamp2 antibody: Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: 1:500- 1:1000 (ICC)
• Conjugate: ATTO 390
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunocytochemistry/Immunofluorescence (ICC/IF)

(Immunocytochemistry/Immunofluorescence analysis using Rat Anti-LAMP2 Monoclonal Antibody, Clone GL2A7. Tissue: Corneal Endothelial Cell (CEC). Species: Rabbit. Primary Antibody: Rat Anti-LAMP2 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green). Courtesy of: Eunduck E.P. Kay, Doheny Eye Institute.)

Western Blot (WB)

(Western Blot analysis of Human, Mouse HEK293 and 3T3NIH cell lysates showing detection of ~100-110 kDa LAMP2 protein using Rat Anti-LAMP2 Monoclonal Antibody, Clone GL2A7. Lane 1: MW ladder. Lane 2: Human HEK293 lysate (20 ug). Lane 3: Mouse 3T3NIH lysate (10 ug). Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rat Anti-LAMP2 Monoclonal Antibody at 1:500 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Rat at 1:100 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~100-110 kDa.)

Related Product Information for anti-Lamp2 antibody

Background Info: Recommended for use in ICC. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin.

Scientific Background: Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). LAMP2 has also been detected at the plasma membrane of cells, as well as in cells that secrete lysosomal hydrolases. A study in the developmental expresses patterns of membrane LAMP2 transcripts indicate a possible involvement of this protein in cell-cell or cell-extracellular matrix interaction, and appear to reflect tissue and cell type specific roles of lysosomes during morphogenesis (4). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl-terminal tyrosine based motif (YXXI) (5). This stimulation has also been shown to have an associated release of histamine, leukotriene C 9$) and prostaglandin D 9@), which shows that LAMP1 and LAMP2 are activation markers for normal mast cells (5). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (6). LAMP2 has also been shown to be critical for autophagy, in conversion of early autophagic vacuoles to vacuoles which rapidly degrade their content (7).

Product Categories/Family for anti-Lamp2 antibody

Chaperones; Organelle Markers; Trafficking

References

1. Granger B.L., et al. (1990) J. Biol. Chem. 265: 12036-12043. 2. Furuta K., et al. (1999) EMBO J. 17(5):1304-14. 3. Rohrer J., et al. (1996) J Cell Biol. 132(4): 565-76. 4. Lichter-Konecki U., et al (1999) Differentiation 65(1): 43-58. 5. Grutzkau A., et al. (2004) Cytometry A. 61(1): 62-68. 6. Kannan K., et al. (1996) Cell Immunol. 171: 10-19. 7. Tanaka Y., et al. (2000) Nature 406: 902-906.

NCBI and Uniprot Product Information

• NCBI GI #: 63054837
• NCBI GeneID: 16784
• NCBI Accession #: NP_001017959.1
• NCBI GenBank Nucleotide #: NM_001017959.2
• UniProt Accession #: P17047; Q3TXG5; Q8BSG8; A2A430
• Molecular Weight: 45,655 Da
• NCBI Official Full Name: lysosome-associated membrane glycoprotein 2 isoform 1
• NCBI Official Synonym Full Names: lysosomal-associated membrane protein 2
• NCBI Official Symbol: Lamp2
• NCBI Official Synonym Symbols: Mac3; LGP-B; CD107b; Lamp-2; Lamp II; Lamp-2a; Lamp-2b; Lamp-2c
• NCBI Protein Information: lysosome-associated membrane glycoprotein 2; lysosomal membrane glycoprotein 2; CD107 antigen-like family member B; lysosomal membrane glycoprotein type B
• UniProt Protein Name: Lysosome-associated membrane glycoprotein 2
• Protein Family: Lysosome-associated membrane glycoprotein
• UniProt Gene Name: Lamp2
• UniProt Synonym Gene Names: Lamp-2; LAMP-2; Lysosome-associated membrane protein 2; LGP-B
• UniProt Entry Name: LAMP2_MOUSE

Uniprot Description

LAMP2: Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. Defects in LAMP2 are the cause of Danon disease (DAND); also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes. Belongs to the LAMP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: phagocytic vesicle membrane; membrane; lysosomal membrane; lysosome; late endosome membrane; late endosome; plasma membrane; integral to membrane; platelet dense granule membrane; endosome

Molecular Function: protein domain specific binding; enzyme binding

Biological Process: protein stabilization