Mouse LAMP1 Monoclonal Antibody | anti-LAMP1 antibody

LAMP1 Antibody

Cat. Num. AAA415183

• Reactivity: Human, cow, pig, mouse, rat and other mammals
• Applications: Immunofluorescence, Western Blot
• Purity: Affinity purified
• Synonyms: LAMP1; Monoclonal Antibody; LAMP1 Antibody; anti-LAMP1 antibody

• Host: Mouse
• Reactivity: Human, cow, pig, mouse, rat and other mammals
• Clonality: Monoclonal
• Isotype: IgG1
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)

• Applicable Applications for anti-LAMP1 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: he antibody solution can be used at dilutions of at least 1:2,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10,000 or lower.
• Immunogen: As the name suggests, LAMP1 is a protein primarily associated with the lysosomal membrane. Antibodies to LAMP1 are therefore excellent markers of lysosomes in mammalian cells, though some LAMP1 may also be seen on late endosomes and on the plasma membrane. In a typical cell LAMP1 is associated with spherical vesicles located next to the nucleus and the microtubule organizing center (1). The protein is also known as CD107a, lysosomal associated membrane glycoprotein 1, LGP120 and LAMPA, as the protein was independently discovered and named by several different labs. CD is an abbreviation for "Cluster of Differentiation" and refers to a protocol for the naming of proteins and other surface markers of membranes of human leucocytes defined by binding of specific monoclonal antibodies. LAMP1 is found on the cell surface of lymphocytes undergoing degranulation, a process in which cytoplasmic vesicles fuse with the plasma membrane, and this phenomena resulted in discovery of LAMP1 as a CD protein. The LAMP1 protein has a large N-terminal region which is inside the lysosome, hence topologically external to the cell, which is often refered to as the lumenal domain (2). The lumenal domain consists of two homologous globular seqments separated by a proline rich sequence. Next there is a single membrane spanning domain and a short 11 amino acid C-terminal cytoplasmic tail. This tail region contains, at the extreme C-terminus, a so-called YXXI motif which is responsible for the sorting of the intact molecule to the endosome and lysozome, where Y = tyrosine, I = isoleucine and X = almost any amino acid (3). This motif is found in several other lysosomal proteins, where it functions in the same way. There are 417 amino acids in the human LAMP1 molecule, giving a native molecular weight of 44.8kDa. However the N-terminal lumenal segment of LAMP1 is very heavily and variably glycosylated due to the presence of 18 N-linked glycosylation sites, so that on SDS-PAGE and on Western blots the protein runs as a diffuse band at 90-120kDa. The HGNC name for this protein is LAMP1.
• Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

References

1. Matteoni, R. and Kreiss, T. E. Translocation and clustering of endosomes and lysosomes depends on microtubules. J. Cell Biol. 105:1253-1265 (1987).2. Howe CL, Granger BL, Hull M, Green SA, Gabel CA, Helenius A, Mellman I. Derived protein sequence, oligosaccharides, and membrane insertion of the 120-kDa lysosomal membrane glycoprotein (lgp120): identification of a highly conserved family of lysosomal membrane glycoproteins. Proc Natl Acad Sci U S A. 85:7577-81 (1988).3. Rohrer J, Schweizer A, Russell D, Kornfeld S. The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane. J Cell Biol. 132:565-76 (1996).