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Western Blot (WB) (Western blot analysis of Lactate Dehydrogenase on different lysates using anti-Lactate Dehydrogenase antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: A549 Lane 3: MCF-7)

Rabbit Lactate Dehydrogenase Monoclonal Antibody | anti-LDHA antibody

Lactate Dehydrogenase Antibody

Gene Names
LDHA; LDH1; LDHM; GSD11; PIG19
Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Lactate Dehydrogenase; Monoclonal Antibody; Lactate Dehydrogenase Antibody; Cell proliferation-inducing gene 19 protein antibody; GSD11 antibody; L lactate dehydrogenase B chain antibody; L-lactate dehydrogenase A chain antibody; Lactate dehydrogenase A antibody; Lactate dehydrogenase B antibody; Lactate dehydrogenase H chain antibody; Lactate dehydrogenase M antibody; LDH A antibody; LDH B antibody; LDH H antibody; LDH heart subunit antibody; LDH M antibody; LDH muscle subunit antibody; LDH-A antibody; LDH-M antibody; LDH1 antibody; ldha antibody; LDHA_HUMAN antibody; LDHBD antibody; LDHM antibody; MS1111 antibody; PIG19 antibody; Proliferation inducing gene 19 antibody; Renal carcinoma antigen NY REN 46 antibody; Renal carcinoma antigen NY-REN-59 antibody; TRG 5 antibody; TRG5 antibody; anti-LDHA antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Zebrafish
Clonality
Monoclonal
Clone Number
SU39-06
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-LDHA antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-5000
IHC: 1:200-1:500
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Lactate Dehydrogenase on different lysates using anti-Lactate Dehydrogenase antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: A549 Lane 3: MCF-7)

Western Blot (WB) (Western blot analysis of Lactate Dehydrogenase on different lysates using anti-Lactate Dehydrogenase antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: A549 Lane 3: MCF-7)

Western Blot (WB)

(Western blot analysis of Lactate Dehydrogenase on hybrid fish (crucian-carp) brain tissue lysate using anti-Lactate Dehydrogenase antibody at 1/500 dilution.)

Western Blot (WB) (Western blot analysis of Lactate Dehydrogenase on hybrid fish (crucian-carp) brain tissue lysate using anti-Lactate Dehydrogenase antibody at 1/500 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining Lactate Dehydrogenase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining Lactate Dehydrogenase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining Lactate Dehydrogenase in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining Lactate Dehydrogenase in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-LDHA antibody
The lactate dehydrogenase family (LDH) catalyzes the final step of anaerobic glycolysis, the conversion of L-lactate and NAD to pyruvate and NADH. The LDH family consists of three members, LDH-A, LDH-B and LDH-C, all of which form tetramers consisting four subunits. However, each family member displays a specific tissue distribution pattern with LDH-A and LDH-B predominant in several tissues, specifically LDH-A in muscle and LDH-B in heart, while LDH-C expression is confined to the testis and sperm. LDHs function as powerful markers for germ cell tumors. The genes encoding human LDH-A and LDH-C map to chromosome 11, while the human LDH-B gene maps to chromosome 12. Deficiency in the LDH-A gene is linked to exertional myoglobinuria.
Product Categories/Family for anti-LDHA antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
332
NCBI Official Full Name
L-lactate dehydrogenase A chain isoform 2
NCBI Official Synonym Full Names
lactate dehydrogenase A
NCBI Official Symbol
LDHA
NCBI Official Synonym Symbols
LDH1; LDHM; GSD11; PIG19
NCBI Protein Information
L-lactate dehydrogenase A chain; LDH-A; LDH-M; LDH muscle subunit; lactate dehydrogenase M; proliferation-inducing gene 19; renal carcinoma antigen NY-REN-59; cell proliferation-inducing gene 19 protein
UniProt Protein Name
L-lactate dehydrogenase A chain
Protein Family
UniProt Gene Name
LDHA
UniProt Synonym Gene Names
LDH-A; LDH-M
UniProt Entry Name
LDHA_HUMAN

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Product Notes

The LDHA ldha (Catalog #AAA9435659) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Lactate Dehydrogenase Antibody reacts with Human, Mouse, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's Lactate Dehydrogenase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-5000 IHC: 1:200-1:500 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the LDHA ldha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lactate Dehydrogenase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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