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Immunohistochemistry (IHC) (Anti-KRT19 / CK19 / Cytokeratin 19 antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Mouse anti-Human KRT19 / CK19 / Cytokeratin 19 Monoclonal Antibody | anti-KRT19 antibody

Mouse Monoclonal [clone AT13D10] (IgG2b,k) to Human KRT19 / CK19 / Cytokeratin 19

Gene Names
KRT19; K19; CK19; K1CS
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Protein A Purified
Synonyms
KRT19 / CK19 / Cytokeratin 19; Monoclonal Antibody; Mouse Monoclonal [clone AT13D10] (IgG2b; k) to Human KRT19 / CK19 / Cytokeratin 19; Anti-KRT19 / CK19 / Cytokeratin 19 Antibody (clone AT13D10) IHC-plus; KRT19; CK19; Cytokeratin 19; Cytokeratin-19; Keratin 19; Keratin-19; CK-19; K19; K1CS; Keratin; type I; 40-kd; Human KRT19; anti-KRT19 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
AT13D10
Specificity
Human KRT19 / CK19 / Cytokeratin 19
Purity/Purification
Protein A Purified
Form/Format
Supplied in PBS, pH 7.4, 10% glycerol, 0.02% sodium azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
400
Applicable Applications for anti-KRT19 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA, IHC-P (10 ug/ml), WB (1:1000)
Target Species
Human
Immunogen Description
Recombinant human KRT19 (1-400aa) purified from E Coli.
Immunogen Type
Recombinant protein
Immunogen
KRT19 / CK19 / Cytokeratin 19 antibody was raised against recombinant human KRT19 (1-400aa) purified from E Coli.
Preparation and Storage
Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

Immunohistochemistry (IHC)

(Anti-KRT19 / CK19 / Cytokeratin 19 antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC) (Anti-KRT19 / CK19 / Cytokeratin 19 antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western Blot: The cell lysates of MCF7 (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT19 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

Western Blot (WB) (Western Blot: The cell lysates of MCF7 (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT19 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-KRT19 antibody
Cytokeratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,106 Da
NCBI Official Full Name
keratin, type I cytoskeletal 19
NCBI Official Synonym Full Names
keratin 19
NCBI Official Symbol
KRT19
NCBI Official Synonym Symbols
K19; CK19; K1CS
NCBI Protein Information
keratin, type I cytoskeletal 19; CK-19; keratin-19; cytokeratin 19; cytokeratin-19; keratin, type I, 40-kd; 40-kDa keratin intermediate filament
UniProt Protein Name
Keratin, type I cytoskeletal 19
Protein Family
UniProt Gene Name
KRT19
UniProt Synonym Gene Names
CK-19; K19
UniProt Entry Name
K1C19_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

Uniprot Description

K19: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 19 differs from all other IF proteins in lacking the C-terminal tail domain. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: dystrophin-associated glycoprotein complex; costamere; intermediate filament; Z disc; sarcolemma

Molecular Function: protein binding; structural constituent of cytoskeleton; structural constituent of muscle; protein complex binding

Biological Process: viral reproduction; response to estrogen stimulus; sarcomere organization

Research Articles on KRT19

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Product Notes

The KRT19 krt19 (Catalog #AAA249135) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone AT13D10] (IgG2b,k) to Human KRT19 / CK19 / Cytokeratin 19 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KRT19 / CK19 / Cytokeratin 19 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA, IHC-P (10 ug/ml), WB (1:1000). Researchers should empirically determine the suitability of the KRT19 krt19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT19 / CK19 / Cytokeratin 19, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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