Mouse anti-Human KRT17 Monoclonal Antibody | anti-KRT17 antibody

KRT17 (Keratin 17, Cytokeratin-17, K17, PC, PC2, PCHC1) (HRP)

Cat. Num. AAA6156065

• Gene Names: KRT17; PC; K17; PC2; 39.1; CK-17; PCHC1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified
• Synonyms: KRT17; Monoclonal Antibody; KRT17 (Keratin 17; Cytokeratin-17; K17; PC; PC2; PCHC1) (HRP); anti-KRT17 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2D10
• Specificity: Recognizes human KRT17.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.4. No preservative added. Labeled with horseradish peroxidase (HRP).
• Sequence Length: 432

• Applicable Applications for anti-KRT17 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full-length recombinant protein corresponding to aa1-432 of human KRT17 with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MTTSIRQFTSSSSIKGSSGLGGGSSRTSCRLSGGLGAGSCRLGSAGGLGSTLGGSSYSSCYSFGSGGGYGSSFGGVDGLLAGGEKATMQNLNDRLASYLDKVRALEEANTELEVKIRDWYQRQAPGPARDYSQYYRTIEELQNKILTATVDNANILLQIDNARLAADDFRTKFETEQALRLSVEADINGLRRVLDELTLARADLEMQIENLKEELAYLKKNHEEEMNALRGQVGGEINVEMDAAPGVDLSRIPNEMRDQYEKMAEKNRKDAEDWFFSKTEELNREVATNSELVQSGKSEISELRRTMQALEIELQSQLSMKASLEGNLAETENRYCVQLSQIQGLIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLTQYKKEPVTTRQVRTIVEEVQDGKVISSREQVHQTTR
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of KRT17 expression in transfected 293T cell line using 207753. Lane 1: KRT17 transfected lysate (48.1kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase to KRT17 on formalin-fixed paraffin-embedded human small Intestine using 207753 (3ug/ml).)

Testing Data

(Detection limit for 207753 is ~3ng/ml as a capture antibody.)

Product Categories/Family for anti-KRT17 antibody

Antibodies; Keratin; Cytokeratin

References

1. Molecular classification of nonsmall-cell lung cancer using a 4-protein quantitative assay. Anagnostou VK, Dimou AT, Botsis T, Killiam EJ, Gustavson MD, Homer RJ, Boffa D, Zolota V, Dougenis D, Tanoue L, Gettinger SN, Detterbeck FC, Syrigos KN, Bepler G, Rimm DL. Cancer. 2012 Mar 15;118(6):1607-18. doi: 10.1002/cncr.26450. Epub 2011 Aug 25.

NCBI and Uniprot Product Information

• NCBI GI #: 14198021
• NCBI GeneID: 3872
• NCBI Official Full Name: Keratin 17
• NCBI Official Synonym Full Names: keratin 17
• NCBI Official Symbol: KRT17
• NCBI Official Synonym Symbols: PC; K17; PC2; 39.1; CK-17; PCHC1
• NCBI Protein Information: keratin, type I cytoskeletal 17
• Protein Family: Keratin

NCBI Description

This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. [provided by RefSeq, Aug 2008]