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Western Blot (WB) (Western blot analysis of BNIP2 expression in HEK293T (A), mouse liver (B), rat liver (C) whole cell lysates.)

Rabbit BNIP2 Polyclonal Antibody | anti-BNIP2 antibody

Anti-BNIP2 Antibody

Gene Names
BNIP2; NIP2; BNIP-2
Reactivity
Human, Mouse, Rat, Bovine
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
BNIP2; Polyclonal Antibody; Anti-BNIP2 Antibody; NIP2; BCL2/adenovirus E1B 19 kDa protein-interacting protein 2; anti-BNIP2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of BNIP2 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
435
Applicable Applications for anti-BNIP2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BNIP2. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of BNIP2 expression in HEK293T (A), mouse liver (B), rat liver (C) whole cell lysates.)

Western Blot (WB) (Western blot analysis of BNIP2 expression in HEK293T (A), mouse liver (B), rat liver (C) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of BNIP2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of BNIP2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-BNIP2 antibody
Rabbit polyclonal antibody to BNIP2

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
663
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,135 Da
NCBI Official Full Name
BCL2/adenovirus E1B 19 kDa protein-interacting protein 2
NCBI Official Synonym Full Names
BCL2/adenovirus E1B 19kDa interacting protein 2
NCBI Official Symbol
BNIP2
NCBI Official Synonym Symbols
NIP2; BNIP-2
NCBI Protein Information
BCL2/adenovirus E1B 19 kDa protein-interacting protein 2
UniProt Protein Name
BCL2/adenovirus E1B 19 kDa protein-interacting protein 2
UniProt Gene Name
BNIP2
UniProt Synonym Gene Names
NIP2
UniProt Entry Name
BNIP2_HUMAN

NCBI Description

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. [provided by RefSeq, Mar 2011]

Uniprot Description

BNIP2: Implicated in the suppression of cell death. Interacts with the BCL-2 and adenovirus E1B 19 kDa proteins. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; nuclear envelope; cytosol

Molecular Function: identical protein binding; protein binding; calcium ion binding; GTPase activator activity

Biological Process: muscle cell differentiation; apoptosis; positive regulation of muscle cell differentiation; negative regulation of apoptosis; positive regulation of GTPase activity

Research Articles on BNIP2

Similar Products

Product Notes

The BNIP2 bnip2 (Catalog #AAA822035) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-BNIP2 Antibody reacts with Human, Mouse, Rat, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's BNIP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000), IH (1/100 - 1/200). Researchers should empirically determine the suitability of the BNIP2 bnip2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BNIP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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