Rat anti-Mouse Klotho Monoclonal Antibody | anti-KL antibody

Klotho (KL)

Cat. Num. AAA603640

• Gene Names: KL; KL
• Reactivity: Mouse
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Klotho; Monoclonal Antibody; Klotho (KL); Anti -Klotho (KL); anti-KL antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 7C39
• Specificity: Recognizes mouse Klotho.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.05% sodium azide, 40% glycerol.

• Applicable Applications for anti-KL antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry.; Dilution: Western Blot: 1-5ug/ml. Using Chemiluminescence technique.; ELISA: 1:10,000-1:100,000. Using 50-100 ng of control peptide/well.; Immunohistochemistry: 2-20ug/ml.
• Immunogen: Purified recombinant mouse klotho protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-KL antibody

Animal models of human aging, which display the characteristic diseases associated with aging, provide insight into the cause of such diseases. Recently a transgenic mouse model, called Klotho, with several premature aging phenotypes has been described. They display premature aging phenotypes such as Osteoporosis, age related skin changes, ectopic calcifications, atrophy of genital organs and thymus, emphysema and short life span (1). KL protein associated with the Klotho mutation is a 1014 amino acid long peptide. It has a putative signal sequence at its N-terminus and a single transmembrane domain near its C-terminus, which is postulated to anchor it to the membrane (2). The extracellular domain has two internal repeats, KL-1 and KL-2 which have 20-40% sequence identity to bacterial b-glucosidase of plants and animals and mammalian lactase glycosylceramidase suggesting a role for Klotho in sphingolipid metabolism (2). kl gene expression was observed to be tissue specific. Improvement of systemic aging phenotypes in kl/kl mice occurs even when the exogenous expression was limited to some organs, suggesting that KL associated aging is regulated through a humoral signaling pathway. KL has been reported to be localized on the cell surface when expressed on Cho cells (1). Human kl cDNA is expected to encode a protein of 1012 amino acids and has 86% homology to mouse kl. Alternative splicing produces two isoforms (a full length membrane bound and the secreted form).

Product Categories/Family for anti-KL antibody

Antibodies; Abs to Enzymes; Glycosidase

NCBI and Uniprot Product Information

• NCBI GI #: 2618596
• NCBI GeneID: 9365
• UniProt Accession #: Q9UEF7; Q5VZ95; Q96KV5; Q96KW5; Q9UEI9; Q9Y4F0
• Molecular Weight: 116,181 Da
• NCBI Official Full Name: klotho
• NCBI Official Synonym Full Names: klotho
• NCBI Official Symbol: KL
• NCBI Official Synonym Symbols: KL
• NCBI Protein Information: klotho; OTTHUMP00000018796
• UniProt Protein Name: Klotho
• Protein Family: Klotho
• UniProt Gene Name: KL
• UniProt Entry Name: KLOT_HUMAN

NCBI Description

This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq]

Uniprot Description

Klotho: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 3.2.1.31; Hydrolase

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; integral to membrane; extracellular region

Molecular Function: vitamin D binding; signal transducer activity; beta-glucuronidase activity; beta-glucosidase activity; fibroblast growth factor binding; hormone activity; fibroblast growth factor receptor binding

Biological Process: calcium ion homeostasis; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; carbohydrate metabolic process; insulin receptor signaling pathway; energy reserve metabolic process; innate immune response; positive regulation of bone mineralization; acute inflammatory response; aging

Disease: Tumoral Calcinosis, Hyperphosphatemic, Familial