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Western Blot (WB) (Western Blot detection against Immunogen (37.11kD).)

Mouse anti-Human KLHL4 Monoclonal Antibody | anti-KLHL4 antibody

KLHL4 (Kelch-like Protein 4, KIAA1687, DKELCHL) (PE)

Gene Names
KLHL4; KHL4; DKELCHL
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
KLHL4; Monoclonal Antibody; KLHL4 (Kelch-like Protein 4; KIAA1687; DKELCHL) (PE); anti-KLHL4 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4B6
Specificity
Recognizes human KLHL4.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-KLHL4 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa1-101 from human KLHL4 (NP_061990) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MSVSGKKEFDVKQILRLRWRWFSHPFQGSTNTGSCLQQEGYEHRGTPVQGRLKSHSRDRNGLKKSNSPVHHNILAPVPGPAPAHQRAVQNLQQHNLIVHF
Conjugate
PE
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (37.11kD).)

Western Blot (WB) (Western Blot detection against Immunogen (37.11kD).)

Western Blot (WB)

(Western Blot analysis of KLHL4 expression in transfected 293T cell line by KLHL4 monoclonal antibody. Lane 1: KLHL4 transfected lysate (80.2kD). Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of KLHL4 expression in transfected 293T cell line by KLHL4 monoclonal antibody. Lane 1: KLHL4 transfected lysate (80.2kD). Lane 2: Non-transfected lysate.)

Testing Data

(Detection limit for recombinant GST tagged KLHL4 is 1ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged KLHL4 is 1ng/ml as a capture antibody.)
Related Product Information for anti-KLHL4 antibody
This gene encodes a member of the kelch family of proteins, which are characterized by kelch repeat motifs and a POZ/BTB protein-binding domain. It is thought that kelch repeats are actin binding domains. However, the specific function of this protein has not been determined. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
Product Categories/Family for anti-KLHL4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
80,835 Da
NCBI Official Full Name
kelch-like protein 4 isoform 1
NCBI Official Synonym Full Names
kelch-like family member 4
NCBI Official Symbol
KLHL4
NCBI Official Synonym Symbols
KHL4; DKELCHL
NCBI Protein Information
kelch-like protein 4
UniProt Protein Name
Kelch-like protein 4
Protein Family
UniProt Gene Name
KLHL4
UniProt Synonym Gene Names
KIAA1687

NCBI Description

This gene encodes a member of the kelch family of proteins, which are characterized by kelch repeat motifs and a POZ/BTB protein-binding domain. It is thought that kelch repeats are actin binding domains. However, the specific function of this protein has not been determined. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

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Product Notes

The KLHL4 klhl4 (Catalog #AAA6158532) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KLHL4 (Kelch-like Protein 4, KIAA1687, DKELCHL) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KLHL4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the KLHL4 klhl4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLHL4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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