Mouse Kir2.3 Monoclonal Antibody | anti-KCNJ4 antibody

Kir2.3 (IRK3, BIR11, Kcnj4)

Cat. Num. AAA608657

• Gene Names: KCNJ4; HIR; HRK1; IRK3; HIRK2; IRK-3; Kir2.3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Kir2.3; Monoclonal Antibody; Kir2.3 (IRK3; BIR11; Kcnj4); Anti -Kir2.3 (IRK3; anti-KCNJ4 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10F748
• Specificity: Recognizes Kir2.3 at ~45kD. Species Crossreactivity: human, rat, mouse. Does not crossreact with Kir2.1 or Kir2.2.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH7.4, 0.09% sodium azide, 50% glycerol.

• Applicable Applications for anti-KCNJ4 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1ug/ml if results are poor, use lysate without boiling, heat at 37ºC for 15 min.; Immunohistochemistry: 0.1-1ug/ml (HRP), 1-10ug/ml (FITC)
• Immunogen: Fusion protein corresponding to aa390-445 of Kir2.3 (NP_032453).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-KCNJ4 antibody

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge,others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or

Product Categories/Family for anti-KCNJ4 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 62020896
• NCBI GeneID: 3761
• UniProt Accession #: P48050; Q14D44
• Molecular Weight: 49,500 Da
• NCBI Official Full Name: KCNJ4 protein, partial
• NCBI Official Synonym Full Names: potassium inwardly-rectifying channel, subfamily J, member 4
• NCBI Official Symbol: KCNJ4
• NCBI Official Synonym Symbols: HIR; HRK1; IRK3; HIRK2; IRK-3; Kir2.3
• NCBI Protein Information: inward rectifier potassium channel 4; inward rectifier K+ channel Kir2.3; inward rectifier K(+) channel Kir2.3; hippocampal inward rectifier potassium channel; potassium channel, inwardly rectifying subfamily J member 4
• UniProt Protein Name: Inward rectifier potassium channel 4
• Protein Family: Inward rectifier potassium channel
• UniProt Gene Name: KCNJ4
• UniProt Synonym Gene Names: IRK3; HIR; IRK-3
• UniProt Entry Name: IRK4_HUMAN

NCBI Description

Several different potassium channels are known to be involved with electrical signaling in the nervous system. One class is activated by depolarization whereas a second class is not. The latter are referred to as inwardly rectifying K+ channels, and they have a greater tendency to allow potassium to flow into the cell rather than out of it. This asymmetry in potassium ion conductance plays a key role in the excitability of muscle cells and neurons. The protein encoded by this gene is an integral membrane protein and member of the inward rectifier potassium channel family. The encoded protein has a small unitary conductance compared to other members of this protein family. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HIR: This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ4 subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Channel, potassium

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: postsynaptic membrane; voltage-gated potassium channel complex; cytoplasmic vesicle membrane; basolateral plasma membrane; plasma membrane; cell junction

Molecular Function: protein binding; inward rectifier potassium channel activity; PDZ domain binding

Biological Process: synaptic transmission; potassium ion import; potassium ion transport