Rabbit anti-Human KCNJ4 Polyclonal Antibody | anti-KCNJ4 antibody

KCNJ4, NT (KCNJ4, IRK3, Inward rectifier potassium channel 4, HIRK2, HRK1, Hippocampal inward rectifier, Inward rectifier K(+) channel Kir2.3, Potassium channel, inwardly rectifying subfamily J member 4) (PE)

Cat. Num. AAA6312576

• Gene Names: KCNJ4; HIR; HRK1; IRK3; HIRK2; IRK-3; Kir2.3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KCNJ4; Polyclonal Antibody; NT (KCNJ4; IRK3; Inward rectifier potassium channel 4; HIRK2; HRK1; Hippocampal inward rectifier; Inward rectifier K(+) channel Kir2.3; Potassium channel; inwardly rectifying subfamily J member 4) (PE); inwardly rectifying subfamily J member 4; anti-KCNJ4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-KCNJ4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KCNJ4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human KCNJ4.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(KCNJ4 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the KCNJ4 antibody detected the KCNJ4 protein (arrow).)

Related Product Information for anti-KCNJ4 antibody

Several different potassium channels are known to be involved with electrical signaling in the nervous system. One class is activated by depolarization whereas a second class is not. The latter are referred to as inwardly rectifying K+ channels, and they have a greater tendency to allow potassium to flow into the cell rather than out of it. This asymmetry in potassium ion conductance plays a key role in the excitability of muscle cells and neurons. The protein encoded by this gene is an integral membrane protein and member of the inward rectifier potassium channel family. The encoded protein has a small unitary conductance compared to other members of this protein family. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-KCNJ4 antibody

Antibodies; Ion Channel

References

Yokoyama, K., et al. Nephron Clin Pract 115 (4), C237-C243 (2010): Yan, X., et al. J. Mol. Biol. 392(4):967-976(2009). He, Y., et al. FEBS Lett. 582(15):2338-2342(2008). Ji, W., et al. Nat. Genet. 40(5):592-599(2008). Ureche, O.N., et al. Cell. Physiol. Biochem. 21 (5-6), 347-356 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 23110984
• NCBI GeneID: 3761
• NCBI Accession #: NP_690607.1; NP_004972.1
• NCBI GenBank Nucleotide #: NM_152868.2
• UniProt Accession #: P48050; Q14D44
• Molecular Weight: 49,500 Da
• NCBI Official Full Name: inward rectifier potassium channel 4
• NCBI Official Synonym Full Names: potassium voltage-gated channel subfamily J member 4
• NCBI Official Symbol: KCNJ4
• NCBI Official Synonym Symbols: HIR; HRK1; IRK3; HIRK2; IRK-3; Kir2.3
• NCBI Protein Information: inward rectifier potassium channel 4
• UniProt Protein Name: Inward rectifier potassium channel 4
• Protein Family: Inward rectifier potassium channel
• UniProt Gene Name: KCNJ4
• UniProt Synonym Gene Names: IRK3; HIR; IRK-3
• UniProt Entry Name: IRK4_HUMAN

NCBI Description

Several different potassium channels are known to be involved with electrical signaling in the nervous system. One class is activated by depolarization whereas a second class is not. The latter are referred to as inwardly rectifying K+ channels, and they have a greater tendency to allow potassium to flow into the cell rather than out of it. This asymmetry in potassium ion conductance plays a key role in the excitability of muscle cells and neurons. The protein encoded by this gene is an integral membrane protein and member of the inward rectifier potassium channel family. The encoded protein has a small unitary conductance compared to other members of this protein family. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HIR: This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ4 subfamily.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, potassium

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: postsynaptic membrane; voltage-gated potassium channel complex; cytoplasmic vesicle membrane; basolateral plasma membrane; plasma membrane; cell junction

Molecular Function: protein binding; inward rectifier potassium channel activity; PDZ domain binding

Biological Process: synaptic transmission; potassium ion import; potassium ion transport