Rabbit anti-Human, Mouse BUB1 Polyclonal Antibody | anti-BUB1 antibody

BUB1 (Mitotic Checkpoint Serine/Threonine-protein Kinase BUB1, hBub1, BUB1A, BUB1L) (HRP)

Cat. Num. AAA6371534

• Gene Names: BUB1; BUB1A; BUB1L; hBUB1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BUB1; Polyclonal Antibody; BUB1 (Mitotic Checkpoint Serine/Threonine-protein Kinase BUB1; hBub1; BUB1A; BUB1L) (HRP); anti-BUB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BUB1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-BUB1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human BUB1, aa1-1085 (NP_004327.1).
• Immunogen Sequence: MDTPENVLQMLEAHMQSYKGNDPLGEWERYIQWVEENFPENKEYLITLLEHLMKEFLDKKKYHNDPRFISYCLKFAEYNSDLHQFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASAVLQRGIQNQAEPREFLQQQYRLFQTRLTETHLPAQARTSEPLHNVQVLNQMITSKSNPGNNMACISKNQGSELSGVISSACDKESNMERRVITISKSEYSVHSSLASKVDVEQVVMYCKEKLIRGESEFSFEELRAQKYNQRRKHEQWVNEDRHYMKRKEANAFEEQLLKQKMDELHKKLHQVVETSHEDLPASQERSEVNPARMGPSVGSQQELRAPCLPVTYQQTPVNMEKNPREAPPVVPPLANAISAALVSPATSQSIAPPVPLKAQTVTDSMFAVASKDAGCVNKSTHEFKPQSGAEIKEGCETHKVANTSSFHTTPNTSLGMVQATPSKVQPSPTVHTKEALGFIMNMFQAPTLPDISDDKDEWQSLDQNEDAFEAQFQKNVRSSGAWGVNKIISSLSSAFHVFEDGNKENYGLPQPKNKPTGARTFGERSVSRLPSKPKEEVPHAEEFLDDSTVWGIRCNKTLAPSPKSPGDFTSAAQLASTPFHKLPVESVHILEDKENVVAKQCTQATLDSCEENMVVPSRDGKFSPIQEKSPKQALSSHMYSASLLRLSQPAAGGVLTCEAELGVEACRLTDTDAAIAEDPPDAIAGLQAEWMQMSSLGTVDAPNFIVGNPWDDKLIFKLLSGLSKPVSSYPNTFEWQCKLPAIKPKTEFQLGSKLVYVHHLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMERLKPSMQHMFMKFYSAHLFQNGSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMRMLYMIEQVHDCEIIHGDIKPDNFILGNGFLEQDDEDDLSAGLALIDLGQSIDMKLFPKGTIFTAKCETSGFQCVEMLSNKPWNYQIDYFGVAATVYCMLFGTYMKVKNEGGECKPEGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKVFQQHYTNKIRALRNRLIVLLLECKRSRK
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(BUB1 rabbit polyclonal antibody. Western Blot analysis of BUB1 expression in mouse testis.)

Western Blot (WB)

(BUB1 rabbit polyclonal antibody. Western Blot analysis of BUB1 expression in Jurkat.)

Western Blot (WB)

(Western Blot analysis of BUB1 expression in transfected 293T cell line by BUB1 polyclonal antibody. Lane 1: BUB1 transfected lysate (122.4kD). Lane 2: Non-transfected lysate.)

Testing Data

(Proximity Ligation Analysis (PLA) of protein-protein interactions between BUB1 and APC. HeLa cells were stained with BUB1 rabbit purified polyclonal 1:1200 and APC mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex)

Related Product Information for anti-BUB1 antibody

This gene encodes a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer.

Product Categories/Family for anti-BUB1 antibody

Antibodies; DNA Repair/Damage

NCBI and Uniprot Product Information

• NCBI GI #: 4757878
• NCBI GeneID: 699
• NCBI Accession #: NP_004327.1
• NCBI GenBank Nucleotide #: NM_004336.2
• UniProt Accession #: O43683; O43430; O43643; O60626; Q53QE4
• Molecular Weight: 122,375 Da
• NCBI Official Full Name: mitotic checkpoint serine/threonine-protein kinase BUB1
• NCBI Official Synonym Full Names: BUB1 mitotic checkpoint serine/threonine kinase
• NCBI Official Symbol: BUB1
• NCBI Official Synonym Symbols: BUB1A; BUB1L; hBUB1
• NCBI Protein Information: mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase; budding uninhibited by benzimidazoles 1 homolog; BUB1 budding uninhibited by benzimidazoles 1 homolog
• UniProt Protein Name: Mitotic checkpoint serine/threonine-protein kinase BUB1
• Protein Family: Checkpoint serine/threonine-protein kinase
• UniProt Gene Name: BUB1
• UniProt Synonym Gene Names: BUB1L; hBUB1
• UniProt Entry Name: BUB1_HUMAN

NCBI Description

This gene encodes a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

BUB1: a mitotic-checkpoint protein kinase of the BUB family. Autophosphorylated when the cells enters mitosis. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage. High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index. LOF mutations seen in two colon cancer cell lines exhibiting chromosomal instability, in several leukemias and lymphomas, and in a pancreatic cancer cell line. BUB1 functions in mitotic spindle assembly, and may cause chromosomal instability. In mouse BRCA2 knockouts, mutations in BUB1 and BUBR1 are frequently seen in the resulting tumors. BUB1 and BUBR1 are overexpressed, but not mutated, in many gastric cancers.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Cell cycle regulation; Other group; BUB family

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: nucleoplasm; kinetochore; membrane; cytoplasm; cytosol; condensed nuclear chromosome, pericentric region

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: cell proliferation; mitosis; viral reproduction; apoptosis; cell division; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; regulation of chromosome segregation; regulation of sister chromatid cohesion; mitotic cell cycle; protein amino acid phosphorylation; chromosome segregation