Mouse KIF3B Monoclonal Antibody | anti-KIF3B antibody

KIF3B (Kinesin Family Member 3B, HH0048, KIAA0359) (Biotin)

Cat. Num. AAA6174640

• Gene Names: KIF3B; FLA8; HH0048; KLP-11
• Applications: Western Blot
• Purity: Purified
• Synonyms: KIF3B; Monoclonal Antibody; KIF3B (Kinesin Family Member 3B; HH0048; KIAA0359) (Biotin); Kinesin Family Member 3B; KIAA0359; anti-KIF3B antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 8H4
• Specificity: Recognizes KIF3B.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-KIF3B antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KIF3B (NP_004789.1, 648aa-747aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MIRPEARYRAENIVLLELDMPSRTTRDYEGPAIAPKVQAALDAALQDEDEIQVDASSFESTANKKSKARPKSGRKSGSSSSSSGTPASQLYPQSRGLVPK
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged KIF3B is 3 ng/ml as a capture antibody.)

Western Blot (WB)

(Western Blot analysis of KIF3B expression in transfected 293T cell line by KIF3B monoclonal antibody (M01), clone 8H4.Lane 1: KIF3B transfected lysate (Predicted MW: 85.1 KDa).Lane 2: Non-transfected lysate.)

Related Product Information for anti-KIF3B antibody

The protein encoded by this gene acts as a heterodimer with kinesin family member 3A to aid in chromosome movement during mitosis and meiosis. The encoded protein is a plus end-directed microtubule motor and can interact with the SMC3 subunit of the cohesin complex. In addition, the encoded protein may be involved in the intracellular movement of membranous organelles. This protein and kinesin family member 3A form the kinesin II subfamily of the kinesin superfamily. [provided by RefSeq]

Product Categories/Family for anti-KIF3B antibody

Antibodies; Motor Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4758646
• NCBI GeneID: 9371
• NCBI Accession #: NP_004789.1
• NCBI GenBank Nucleotide #: NM_004798.3
• UniProt Accession #: O15066; B2RMP4; E1P5M5
• Molecular Weight: 85,125 Da
• NCBI Official Full Name: kinesin-like protein KIF3B
• NCBI Official Synonym Full Names: kinesin family member 3B
• NCBI Official Symbol: KIF3B
• NCBI Official Synonym Symbols: FLA8; HH0048; KLP-11
• NCBI Protein Information: kinesin-like protein KIF3B; microtubule plus end-directed kinesin motor 3B
• UniProt Protein Name: Kinesin-like protein KIF3B
• Protein Family: Kinesin-like protein
• UniProt Gene Name: KIF3B
• UniProt Synonym Gene Names: KIAA0359
• UniProt Entry Name: KIF3B_HUMAN

NCBI Description

The protein encoded by this gene acts as a heterodimer with kinesin family member 3A to aid in chromosome movement during mitosis and meiosis. The encoded protein is a plus end-directed microtubule motor and can interact with the SMC3 subunit of the cohesin complex. In addition, the encoded protein may be involved in the intracellular movement of membranous organelles. This protein and kinesin family member 3A form the kinesin II subfamily of the kinesin superfamily. [provided by RefSeq, Jul 2008]

Uniprot Description

KIF3B: Involved in tethering the chromosomes to the spindle pole and in chromosome movement. Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Belongs to the kinesin-like protein family. Kinesin II subfamily.

Protein type: Motor; Microtubule-binding

Chromosomal Location of Human Ortholog: 20q11.21

Cellular Component: plus-end kinesin complex; microtubule cytoskeleton; centrosome; membrane; spindle microtubule; kinesin II complex; midbody; cytosol

Molecular Function: protein binding; Rho GTPase binding; plus-end-directed microtubule motor activity; microtubule binding; ATPase activity; microtubule motor activity; ATP binding

Biological Process: positive regulation of cytokinesis; mitotic centrosome separation; mitotic spindle organization and biogenesis; metabolic process; organelle organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; cytoskeleton-dependent intracellular transport; determination of left/right symmetry; anterograde axon cargo transport; blood coagulation; microtubule-based movement