Mouse anti-Cow, Human Ki-67 Monoclonal Antibody | anti-Ki-67 antibody

Anti-Ki-67 Purified

Cat. Num. AAA1800828

• Gene Names: MKI67; KIA; MIB-; MIB-1; PPP1R105
• Reactivity: Cow, Human
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: Ki-67; Monoclonal Antibody; Anti-Ki-67 Purified; marker of proliferation Ki-67; KIA; MIB; Marker of proliferation Ki67; MKI67; anti-Ki-67 antibody

• Host: Mouse
• Reactivity: Cow, Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: Ki-67
• Specificity: The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 2896

• Applicable Applications for anti-Ki-67 antibody: Flow Cytometry (FC/FACS), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: FC/FACS: QC tested
• Immunogen: Nuclei of the Hodgkin lymphoma cell line L428
• Preparation and Storage: Store at 2-8 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry detection of Ki-67 in U2OS cell line (human osteosarcoma) using monoclonal antibody Ki-67 (green). Cell nuclei stained with DAPI (blue).)

Related Product Information for anti-Ki-67 antibody

Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor identification and is commonly used as a prognostic factor in cancer studies.

NCBI and Uniprot Product Information

• NCBI GI #: 225543215
• NCBI GeneID: 4288
• NCBI Accession #: NP_001139438.1
• NCBI GenBank Nucleotide #: NP_001139438.1
• UniProt Accession #: P46013
• NCBI Official Full Name: proliferation marker protein Ki-67 isoform 2
• NCBI Official Synonym Full Names: marker of proliferation Ki-67
• NCBI Official Symbol: MKI67
• NCBI Official Synonym Symbols: KIA; MIB-; MIB-1; PPP1R105
• NCBI Protein Information: proliferation marker protein Ki-67
• UniProt Protein Name: Antigen KI-67
• UniProt Gene Name: MKI67
• UniProt Entry Name: KI67_HUMAN

NCBI Description

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

Uniprot Description

KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers.

Protein type: Nucleolus; Cell cycle regulation

Chromosomal Location of Human Ortholog: 10q26.2

Cellular Component: membrane; cytoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region

Molecular Function: protein C-terminus binding; protein binding; ATP binding

Biological Process: cell proliferation; meiosis