Mouse anti-Human Ki-67 Monoclonal Antibody | anti-Ki-67 antibody

Ki-67 Antibody

Cat. Num. AAA346016

• Gene Names: Mki67; Ki67; Ki-67; D630048A14Rik
• Reactivity: Human
• Applications: Immunohistochemistry
• Synonyms: Ki-67; Monoclonal Antibody; Ki-67 Antibody; anti-Ki-67 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: IHC067
• Form/Format: Liquid
• Sequence Length: 2938

• Applicable Applications for anti-Ki-67 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Dilution Range: 1:100 - 1:200
• Buffer: Tris Buffer, ph7.4, 1% BSA, <0.1% NaN3
• IHC Positive Control: Tonsil
• IHC Suggested Protocol: 1. Tissues are fixed with 10% neutral buffered formalin, cut to ~4um, and placed on a positively charged glass slide.; 2. The prepared slide is baked for at least 30 minutes and at most 24 hours at 55-65 degree C.; 3. Pretreatment - Perform heat-induced epitope retrieval (HIER) at pH 6 for 10 to 30 minutes.; 4. Block – Block with peroxidase solution for 5 minutes at RT.; 5. Primary Ab – Dilute antibodies 1:100 – 1:200 and incubate for 10 - 30 minutes at RT.; 6. Secondary Ab - Incubate for 20 - 30 minutes at RT.; 7. Substrate - Incubate DAB or Fast Red for 5 - 10 minutes at RT.; 8. Counterstain - Counterstain with hematoxylin for 30 seconds to 5 minutes, rinse with distilled water and blueing solution for 30 seconds.; 9. Dehydrate and apply coverslip.; (Each researcher should optimize the protocol for their experimental model system.)
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.

Immunohistochemistry (IHC)

(IHC (paraffin) of human cervical cancer using Ki-67 Antibody)

Related Product Information for anti-Ki-67 antibody

The Ki-67 protein is a cellular marker that is expressed in the nucleus only during the G1, S, G2, and M phases of the cell cyle and not in the resting phases (G0 and G1). Thus, it is often used as a marker for cell proliferation, and can act as an effective indicator for grading tumor proliferation rates.

NCBI and Uniprot Product Information

• NCBI GI #: 1177528
• NCBI GeneID: 17345
• UniProt Accession #: Q61769; Q7TSF6; E9PVX6
• Molecular Weight: 324,718 Da
• NCBI Official Full Name: Ki-67
• NCBI Official Synonym Full Names: antigen identified by monoclonal antibody Ki 67
• NCBI Official Symbol: Mki67
• NCBI Official Synonym Symbols: Ki67; Ki-67; D630048A14Rik
• NCBI Protein Information: proliferation marker protein Ki-67; antigen KI-67
• UniProt Protein Name: Proliferation marker protein Ki-67
• UniProt Gene Name: Mki67
• UniProt Synonym Gene Names: Antigen KI-67 homologCurated; Antigen Ki67 homologCurated

Uniprot Description

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (). Does not contribute to the internal structure of mitotic chromosomes (PubMed:26949251). May play a role in chromatin organization (PubMed:26949251). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.