Western Blot (WB)
(Western Blot analysis in A431 lysate was resolved by electrophoresis, transferred to PVDF and probed with K1700-09 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates cell-cycle associated protein of 345kD and 395kD.)
Mouse anti-Human Ki-67 Monoclonal Antibody | anti-Ki-67 antibody
Ki-67 (Antigen Identified by Monoclonal Antibody Ki-67, KIA, MKI67, Proliferation Related Ki-67 Antigen) (MaxLight 650)
Gene Names
MKI67; KIA; MIB-; MIB-1; PPP1R105
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Ki-67; Monoclonal Antibody; Ki-67 (Antigen Identified by Monoclonal Antibody Ki-67; KIA; MKI67; Proliferation Related Ki-67 Antigen) (MaxLight 650); anti-Ki-67 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2Q476
Specificity
Recognizes a cell-cycle-associated protein of 345kD and 395kD identical with the human Ki-67 antigen. Binds to a formalin-resistant epitope of the Ki-67 antigen. Reactivity is confined to the nuclei of proliferating cells and no crossreactivity with cytoplasmic antigens of epithelial occurs even after antigen retrieval. Immunohistochemistry labeling of fresh and frozen samples showed identical results with Ki-67 and Ki-S5.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
Sequence Length
12684
Applicable Applications for anti-Ki-67 antibody
Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB)
Immunogen
Nuclear protein preparation from the human cell line U937.50.
Conjugate
MaxLight650
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Western Blot (WB)
(Western Blot analysis in A431 lysate was resolved by electrophoresis, transferred to PVDF and probed with K1700-09 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates cell-cycle associated protein of 345kD and 395kD.)
Western Blot (WB)
(Western Blot analysis in A431 lysate was resolved by electrophoresis, transferred to PVDF and probed with K1700-09 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates cell-cycle associated protein of 345kD and 395kD.)
Western Blot (WB)
(Western Blot analysis in A431 (Lane 1) and C2C12 lysate (Lane 2) were resolved by electrophoresis, transferred to PVDF and probed with K1700-09 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates cell-cycle-associated protein of 345kD and 395kD (~345 and 395kD).)
Western Blot (WB)
(Western Blot analysis in A431 (Lane 1) and C2C12 lysate (Lane 2) were resolved by electrophoresis, transferred to PVDF and probed with K1700-09 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates cell-cycle-associated protein of 345kD and 395kD (~345 and 395kD).)
Immunofluorescence (IF)
(Confolcal fluorescent analysis of HeLa (left) and A431 cells (right) using K1700-09 (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Positive nuclear staining.)
Immunofluorescence (IF)
(Confolcal fluorescent analysis of HeLa (left) and A431 cells (right) using K1700-09 (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Positive nuclear staining.)
Product Categories/Family for anti-Ki-67 antibody
References
1. Kreipe, H., et al., Am. J. Pathol. 142: 1689 (1993). 2. Mauri, F.A., et al., Applied Immunohistochemistry 2: 171 (1984). 3. Ballin, K., et al., Pathology Research and Practice 190: 269 (1994).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens antigen identified by monoclonal antibody Ki-67 (MKI67), mRNA
NCBI Official Synonym Full Names
marker of proliferation Ki-67
NCBI Official Symbol
MKI67
NCBI Official Synonym Symbols
KIA; MIB-; MIB-1; PPP1R105
NCBI Protein Information
proliferation marker protein Ki-67
NCBI Description
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
Research Articles on Ki-67
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Product Notes
The Ki-67 (Catalog #AAA6246527) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Ki-67 (Antigen Identified by Monoclonal Antibody Ki-67, KIA, MKI67, Proliferation Related Ki-67 Antigen) (MaxLight 650) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ki-67 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the Ki-67 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ki-67, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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