Mouse anti-Human Keratin 5 &14 Monoclonal Antibody | anti-KRT14 antibody

Keratin 5 &14

Cat. Num. AAA395184

• Gene Names: KRT14; K14; NFJ; CK14; EBS3; EBS4
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A/G Chromatography
• Synonyms: Keratin 5 &14; Monoclonal Antibody; anti-KRT14 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 16.4
• Purity/Purification: Protein A/G Chromatography
• Form/Format: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Sequence Length: 472

• Applicable Applications for anti-KRT14 antibody: Western Blot (WB), Immunohistochemistry (IHC) Frozen
• Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with cytokeratin enriched extract of feline tongue epithelium and mouse myeloma cells.
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Related Product Information for anti-KRT14 antibody

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.

Product Categories/Family for anti-KRT14 antibody

Monoclonal Antibody; Structural Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 15431310
• NCBI GeneID: 3861
• NCBI Accession #: NP_000517.2
• NCBI GenBank Nucleotide #: NM_000526.4
• UniProt Accession #: P02533; Q14715; Q53XY3; Q9BUE3; Q9UBN2; Q9UBN3; Q9UCY4
• Molecular Weight: 51,561 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 14
• NCBI Official Synonym Full Names: keratin 14
• NCBI Official Symbol: KRT14
• NCBI Official Synonym Symbols: K14; NFJ; CK14; EBS3; EBS4
• NCBI Protein Information: keratin, type I cytoskeletal 14; CK-14; keratin-14; cytokeratin 14; cytokeratin-14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
• UniProt Protein Name: Keratin, type I cytoskeletal 14
• Protein Family: Keratin
• UniProt Gene Name: KRT14
• UniProt Synonym Gene Names: CK-14; K14
• UniProt Entry Name: K1C14_HUMAN

NCBI Description

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. Ref.13

Subunit structure: Heterotetramer of two type I and two type II keratins. disulfide-linked keratin-14 associates with keratin-5. Interacts with TRADD and with keratin filaments. Associates with other type I keratins. Ref.12 Ref.13 Ref.16

Subcellular location: Cytoplasm. Nucleus. Note: Expressed in both as a filamentous pattern. Ref.13 Ref.16

Tissue specificity: Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen. Ref.11

Post-translational modification: A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus.

Involvement in disease: Epidermolysis bullosa simplex, Dowling-Meara type (DM-EBS) [MIM:131760]: A severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.Note: The disease is caused by mutations affecting the gene represented in this entry.Epidermolysis bullosa simplex, Weber-Cockayne type (WC-EBS) [MIM:131800]: A form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.Note: The disease is caused by mutations affecting the gene represented in this entry.Epidermolysis bullosa simplex, Koebner type (K-EBS) [MIM:131900]: A form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, although it is less severe.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.9 Ref.10 Ref.17 Ref.19 Ref.28 Ref.29 Ref.31 Ref.32 Ref.33 Ref.41Epidermolysis bullosa simplex, autosomal recessive (AREBS) [MIM:601001]: An intraepidermal epidermolysis bullosa characterized by localized blistering on the dorsal, lateral and plantar surfaces of the feet.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.21Naegeli-Franceschetti-Jadassohn syndrome (NFJS) [MIM:161000]: A rare autosomal dominant form of ectodermal dysplasia. The cardinal features are absence of dermatoglyphics (fingerprints), reticular cutaneous hyperpigmentation (starting at about the age of 2 years without a preceding inflammatory stage), palmoplantar keratoderma, hypohidrosis with diminished sweat gland function and discomfort provoked by heat, nail dystrophy, and tooth enamel defects.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.39Dermatopathia pigmentosa reticularis (DPR) [MIM:125595]: A rare ectodermal dysplasia characterized by lifelong persistent reticulate hyperpigmentation, non-cicatricial alopecia, and nail dystrophy. Variable features include adermatoglyphia, hypohidrosis or hyperhidrosis, and palmoplantar hyperkeratosis.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.39

Miscellaneous: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Sequence similarities: Belongs to the intermediate filament family.