Rabbit anti-Human C2orf49 Polyclonal Antibody | anti-C2orf49 antibody

C2orf49, NT (C2orf49, Ashwin) (Azide free) (HRP)

Cat. Num. AAA6279829

• Gene Names: C2orf49; asw
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: C2orf49; Polyclonal Antibody; NT (C2orf49; Ashwin) (Azide free) (HRP); Ashwin; anti-C2orf49 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-C2orf49 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: C2orf49 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-100 amino acids from the N-terminal region of human C2orf49.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of C2orf49 Antibody (N-term) in K562 cell line lysates (35ug/lane).C2orf49 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(C2orf49 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C2orf49 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(C2orf49 Antibody (N-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-C2orf49 antibody

The function of this protein has not been specifically defined.

Product Categories/Family for anti-C2orf49 antibody

Antibodies; KIAA; orf

References

Olsen, J.V., et al. Cell 127(3):635-648(2006). Hillier, L.W., et al. Nature 434(7034):724-731(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 12654927
• NCBI GeneID: 79074
• UniProt Accession #: Q9BVC5; B3KXN3
• Molecular Weight: 25,858 Da
• NCBI Official Full Name: Chromosome 2 open reading frame 49
• NCBI Official Synonym Full Names: chromosome 2 open reading frame 49
• NCBI Official Symbol: C2orf49
• NCBI Official Synonym Symbols: asw
• NCBI Protein Information: ashwin
• UniProt Protein Name: Ashwin
• UniProt Gene Name: C2orf49
• UniProt Entry Name: ASHWN_HUMAN

Uniprot Description

Subunit structure: Component of the tRNA-splicing ligase complex. Ref.10

Sequence similarities: Belongs to the ashwin family.