Western Blot (WB)
(Figure 1. Western blot analysis of KAT9/ELP3 using anti-KAT9/ELP3 antibody (MBS1754015).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: human U20S whole cell lysatesLane 3: human HepG2 whole cell lysatesLane 4: human U87 whole cell lysatesLane 5: rat lung tissue lysatesLane 6: rat PC-12 whole cell lysatesLane 7: mouse lung tissue lysatesLane 8: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- KAT9/ELP3 antigen affinity purified monoclonal antibody (Catalog # MBS1754015) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for KAT9/ELP3 at approximately 62KD. The expected band size for KAT9/ELP3 is at 62KD. )
Mouse KAT9/ELP3 Monoclonal Antibody | anti-ELP3 antibody
Anti-KAT9/ELP3 Antibody (monoclonal, 8B4)
Western Blot (WB)
(Figure 1. Western blot analysis of KAT9/ELP3 using anti-KAT9/ELP3 antibody (MBS1754015).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: human U20S whole cell lysatesLane 3: human HepG2 whole cell lysatesLane 4: human U87 whole cell lysatesLane 5: rat lung tissue lysatesLane 6: rat PC-12 whole cell lysatesLane 7: mouse lung tissue lysatesLane 8: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- KAT9/ELP3 antigen affinity purified monoclonal antibody (Catalog # MBS1754015) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for KAT9/ELP3 at approximately 62KD. The expected band size for KAT9/ELP3 is at 62KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of KAT9/ELP3 using anti-KAT9/ELP3 antibody (MBS1754015).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: human U20S whole cell lysatesLane 3: human HepG2 whole cell lysatesLane 4: human U87 whole cell lysatesLane 5: rat lung tissue lysatesLane 6: rat PC-12 whole cell lysatesLane 7: mouse lung tissue lysatesLane 8: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- KAT9/ELP3 antigen affinity purified monoclonal antibody (Catalog # MBS1754015) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for KAT9/ELP3 at approximately 62KD. The expected band size for KAT9/ELP3 is at 62KD. )
2. Creppe C, Malinouskaya L, Volvert ML, Gillard M, Close P, Malaise O, Laguesse S, Cornez I, Rahmouni S, Ormenese S, Belachew S, Malgrange B, Chapelle JP, Siebenlist U, Moonen G, Chariot A, Nguyen L (Feb 2009). "Elongator controls the migration and differentiation of cortical neurons through acetylation of alpha-tubulin". Cell. 136 (3): 551-64.
NCBI and Uniprot Product Information
NCBI Description
ELP3 is the catalytic subunit of the histone acetyltransferase elongator complex, which contributes to transcript elongation and also regulates the maturation of projection neurons (Creppe et al., 2009 [PubMed 19185337]).[supplied by OMIM, Apr 2009]
Uniprot Description
ELP3: a histone acetyltransferase. A subunit of the Elongator complex, which associates with the elongating, hyperphosphorylated form of RNA polymerase II.
Protein type: EC 2.3.1.48; Acetyltransferase
Chromosomal Location of Human Ortholog: 8p21.1
Cellular Component: transcription elongation factor complex; Elongator holoenzyme complex; cytoplasm; nucleolus; histone acetyltransferase complex
Molecular Function: protein binding; H4 histone acetyltransferase activity; metal ion binding; iron-sulfur cluster binding; phosphorylase kinase regulator activity; H3 histone acetyltransferase activity
Biological Process: tRNA wobble uridine modification; regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; central nervous system development; RNA elongation from RNA polymerase II promoter; neuron migration; regulation of protein kinase activity; positive regulation of cell migration
Research Articles on ELP3
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Product Notes
The ELP3 elp3 (Catalog #AAA1754015) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-KAT9/ELP3 Antibody (monoclonal, 8B4) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KAT9/ELP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 0.1-0.25ug/ml|Human, Mouse, Rat|. Researchers should empirically determine the suitability of the ELP3 elp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KAT9/ELP3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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